Document Detail


Signal transduction pathway of nitric oxide inducing PC12 cell death.
MedLine Citation:
PMID:  11834151     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study signal transduction pathway of nitric oxide inducing death of PC12 cells. METHODS: Cell survival rate was measured with MTT assay, and caspase-3 activity with caspase-3 assay kits after PC12 cells were incubated with sodium nitroprusside (SNP), caspase-3 inhibitor II plus SNP or p38 inhibitor-SB203580 plus SNP. RESULTS: SNP induced death of PC12 cells in dose- and time-dependent manner and enhanced caspase-3 activity gradually. Both caspase-3 inhibitor II and SB203580 reduced cell death, but SB203580 reduced caspase-3 activity significantly. CONCLUSIONS: NO may induce death of PC12 cells through activation of p38 and caspase-3.
Authors:
C Luo; R Xu; Z Yang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese journal of traumatology = Zhonghua chuang shang za zhi / Chinese Medical Association     Volume:  4     ISSN:  1008-1275     ISO Abbreviation:  Chin. J. Traumatol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2002-02-08     Completed Date:  2002-03-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  100886162     Medline TA:  Chin J Traumatol     Country:  China    
Other Details:
Languages:  eng     Pagination:  86-8     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou, 510282, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Cells, Cultured
Nitric Oxide / physiology*
PC12 Cells*
Rats
Signal Transduction*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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