Document Detail

Signal transduction alterations in peripheral nerves from streptozotocin-induced diabetic rats.
MedLine Citation:
PMID:  7473883     Owner:  NLM     Status:  MEDLINE    
We have previously determined the presence of muscarinic receptors and the expression of several G proteins in homogenates and myelin fractions from rat sciatic nerves. In the present study we investigated whether changes in several signal transduction pathways in peripheral nerves might be responsible for some of the biochemical abnormalities (e.g., phosphoinositide metabolism) present in sciatic nerves from streptozotocin-induced diabetic rats. Sciatic nerves from 5 week diabetic rats that were prelabelled with [3H]-myo-inositol displayed a significant increase in the basal release of inositol mono- and bis-phosphate, while carbamylcholine-stimulated release was significantly smaller. Basal- and forskolin-stimulated adenylyl cyclase activity was significantly decreased in sciatic nerve homogenates from diabetic animals. However, we were unable to detect any significant differences in the levels of cAMP in intact nerves or in nerve segments that were incubated in the presence or absence of forskolin. ADP-ribosylation experiments showed that in sciatic nerves from experimentally diabetic rats there was a significant increase in the ADP-ribosylation catalyzed by cholera and pertussis toxins. Measurements of the levels of alpha-subunits of G proteins revealed that the expression of Gq/11 alpha, Gs alpha, and Gi-3 alpha was increased by 30 to 50%. These results indicate that during the course of experimental diabetes, peripheral nerves exhibit an abnormal production of inositol phosphates and cAMP, together with an abnormal expression and/or function of G proteins. One of the consequences of such alterations is the diminished release of inositol phosphates triggered by muscarinic agonists in diabetic sciatic nerves.
T Y Goraya; P Wilkins; J G Douglas; J Zhou; L N Berti-Mattera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  41     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-11-24     Completed Date:  1995-11-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  518-25     Citation Subset:  IM    
Department of Medicine, University Hospitals of Cleveland, Ohio, USA.
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MeSH Terms
Adenylate Cyclase / metabolism
Cyclic AMP / metabolism
Diabetes Mellitus, Experimental
Disease Models, Animal
Forskolin / pharmacology
Peripheral Nervous System
Rats, Sprague-Dawley
Sciatic Nerve / metabolism*
Signal Transduction*
Streptozocin / pharmacology*
Grant Support
Reg. No./Substance:
18883-66-4/Streptozocin; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC Cyclase

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