| Signal-boosted qualitative ultrasensitive p24 antigen assay for diagnosis of subtype C HIV-1 infection in infants under the age of 2 years. | |
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MedLine Citation:
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PMID: 16010158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gold standard for diagnosis of HIV-1 infection in infants under the age of 2 years is DNA or reverse transcriptase polymerase chain reaction. However, these tests are expensive and therefore not available in resource-limited countries. With the increasing availability of antiretroviral drugs for prevention of mother-to-child transmission of HIV and treatment of AIDS in resource-poor countries, there is an urgent need to develop cheaper, alternative, and cost-effective laboratory methods for early diagnosis of infant HIV-1 infection that will be useful in identifying infected infants who may benefit from early cotrimoxazole prophylaxis or commencement of antiretroviral therapy. We evaluated an alternative method, the enzyme-linked immunosorbent assay-based qualitative ultrasensitive p24 antigen assay for diagnosis of subtype C HIV-1 infection in infants under the age of 2 years using DNA polymerase chain reaction as the reference method. The assay showed a sensitivity of 96.7% (95% CI: 93.0-100) for detection of HIV-1 infection among infants 0-18 months of age with a specificity of 96.1% (95% CI: 91.7-100). These evaluated parameters were not statistically different between infants aged 0-6 and 7-18 months. The ultrasensitive p24 antigen assay is a useful diagnostic test for detection of HIV-1 infection among infants aged 0-18 months. |
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Authors:
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Lynn S Zijenah; Ocean Tobaiwa; Simbarashe Rusakaniko; Kusum J Nathoo; Margaret Nhembe; Petronella Matibe; David A Katzenstein |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of acquired immune deficiency syndromes (1999) Volume: 39 ISSN: 1525-4135 ISO Abbreviation: J. Acquir. Immune Defic. Syndr. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-12 Completed Date: 2005-09-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100892005 Medline TA: J Acquir Immune Defic Syndr Country: United States |
Other Details:
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Languages: eng Pagination: 391-4 Citation Subset: IM; X |
Affiliation:
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Department of Immunology, Division of Infectious Diseases, College of Health Sciences, University of Zimbabwe, Harare. LZIJENAH@HEALTHNET.ZW |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzyme-Linked Immunosorbent Assay
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methods* HIV Core Protein p24 / blood* HIV Infections / diagnosis*, virology* HIV-1 / classification* Humans Infant Infant, Newborn Sensitivity and Specificity Zimbabwe |
| Chemical | |
Reg. No./Substance:
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0/HIV Core Protein p24 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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