Document Detail


Signal-boosted qualitative ultrasensitive p24 antigen assay for diagnosis of subtype C HIV-1 infection in infants under the age of 2 years.
MedLine Citation:
PMID:  16010158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gold standard for diagnosis of HIV-1 infection in infants under the age of 2 years is DNA or reverse transcriptase polymerase chain reaction. However, these tests are expensive and therefore not available in resource-limited countries. With the increasing availability of antiretroviral drugs for prevention of mother-to-child transmission of HIV and treatment of AIDS in resource-poor countries, there is an urgent need to develop cheaper, alternative, and cost-effective laboratory methods for early diagnosis of infant HIV-1 infection that will be useful in identifying infected infants who may benefit from early cotrimoxazole prophylaxis or commencement of antiretroviral therapy. We evaluated an alternative method, the enzyme-linked immunosorbent assay-based qualitative ultrasensitive p24 antigen assay for diagnosis of subtype C HIV-1 infection in infants under the age of 2 years using DNA polymerase chain reaction as the reference method. The assay showed a sensitivity of 96.7% (95% CI: 93.0-100) for detection of HIV-1 infection among infants 0-18 months of age with a specificity of 96.1% (95% CI: 91.7-100). These evaluated parameters were not statistically different between infants aged 0-6 and 7-18 months. The ultrasensitive p24 antigen assay is a useful diagnostic test for detection of HIV-1 infection among infants aged 0-18 months.
Authors:
Lynn S Zijenah; Ocean Tobaiwa; Simbarashe Rusakaniko; Kusum J Nathoo; Margaret Nhembe; Petronella Matibe; David A Katzenstein
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of acquired immune deficiency syndromes (1999)     Volume:  39     ISSN:  1525-4135     ISO Abbreviation:  J. Acquir. Immune Defic. Syndr.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892005     Medline TA:  J Acquir Immune Defic Syndr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  391-4     Citation Subset:  IM; X    
Affiliation:
Department of Immunology, Division of Infectious Diseases, College of Health Sciences, University of Zimbabwe, Harare. LZIJENAH@HEALTHNET.ZW
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MeSH Terms
Descriptor/Qualifier:
Enzyme-Linked Immunosorbent Assay / methods*
HIV Core Protein p24 / blood*
HIV Infections / diagnosis*,  virology*
HIV-1 / classification*
Humans
Infant
Infant, Newborn
Sensitivity and Specificity
Zimbabwe
Chemical
Reg. No./Substance:
0/HIV Core Protein p24

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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