| Sickling of red blood cells through rapid oxygen exchange in microfluidic drops. | |
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MedLine Citation:
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PMID: 20603684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a microfluidic approach to study the sickling of red blood cells associated with sickle cell anemia by rapidly varying the oxygen partial pressure within flowing microdroplets. By using the perfluorinated carrier oil as a sink or source of oxygen, the oxygen level within the water droplets quickly equilibrates through exchange with the surrounding oil. This provides control over the oxygen partial pressure within an aqueous drop ranging from 1 kPa to ambient partial pressure, i.e. 21 kPa. The dynamics of the oxygen exchange is characterized through fluorescence lifetime measurements of a ruthenium compound dissolved in the aqueous phase. The gas exchange is shown to occur primarily during and directly after droplet formation, in 0.1 to 0.5 s depending on the droplet diameter and speed. The controlled deoxygenation is used to trigger the polymerization of hemoglobin within sickle red blood cells, encapsulated in drops. This process is observed using polarization microscopy, which yields a robust criterion to detect polymerization based on transmitted light intensity through crossed polarizers. |
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Authors:
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Paul Abbyad; Pierre-Louis Tharaux; Jean-Louis Martin; Charles N Baroud; Antigoni Alexandrou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-06 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-07 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 2505-12 Citation Subset: IM |
Affiliation:
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Laboratoire d'Optique et Biosciences (LOB), Ecole Polytechnique, INSERM U696, CNRS, 91128 Palaiseau, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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instrumentation* Cells, Cultured Equipment Design Equipment Failure Analysis Erythrocytes / drug effects, metabolism* Flow Injection Analysis / instrumentation* Hemoglobin, Sickle / drug effects, metabolism* Humans Microfluidic Analytical Techniques / instrumentation* Oxygen / administration & dosage*, pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobin, Sickle; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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