Document Detail


Sialic acids in the chicken anterior lingual glands detected by lectins combined with enzymatic degradation and oxidizing agents.
MedLine Citation:
PMID:  8607294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chicken anterior lingual glands contain a large amount of sialoglycoconjugates. This study is aimed at discriminating between them, on the basis of the degree of acetylation and the acceptor sugars, by means of lectins combined with neuraminidase digestion and treatment with oxidizing and deacetylating agents. In the anteromedial part of lingual glands, only low concentrations of periodate-labile sialic acid having alpha 2-3,6-galactose and alpha 2-6-N-acetylgalactosamine acceptor sugars were present. Mostly, C7 and/or C8 acetylated sialic acids occurred, while C9 acetylated sialic acid linked alpha 2-3-galactose and alpha 2-6-N-acetylgalactosamine were restricted to some cells. In the anterolateral part, sialic acid linked alpha 2-6-N-acetylgalactosamine was not acetylated, while sialoderivatives alpha 2-3 linked to galactose were mainly C9 acetylated.
Authors:
P Scocco; P Ceccarelli; G Menghi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta histochemica     Volume:  97     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-05-20     Completed Date:  1996-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  435-43     Citation Subset:  IM    
Affiliation:
Faculty of Veterinary Medicine, University of Camerino, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Acetylgalactosamine / metabolism
Animals
Binding Sites
Chickens
Female
Galactose / metabolism
Immunohistochemistry
Lectins / metabolism*
Lingual Frenum / chemistry*,  metabolism
Male
N-Acetylneuraminic Acid
Neuraminidase / metabolism
Oxidation-Reduction
Periodic Acid / chemistry
Salivary Glands, Minor / chemistry*,  metabolism
Sialic Acids / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Lectins; 0/Sialic Acids; 10450-60-9/Periodic Acid; 131-48-6/N-Acetylneuraminic Acid; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; 7790-28-5/metaperiodate; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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