| Sialic acid-specific lectin from Tritrichomonas foetus. | |
| | |
MedLine Citation:
|
PMID: 10366765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A novel sialic acid-specific lectin (TFL) was isolated from Tritrichomonas foetus culture supernatant and purified by erythrocyte adsorption followed by fetuin-agarose affinity chromatography. According to gel filtration TFL is a protein of 728 kDa, different from the two sialidases of 853 and 254 kDa, secreted by T. foetus into the medium. The lectin is formed by multimeric complexes of 66 kDa subunit according to SDS-PAGE under reducing conditions. TFL is glycosylated with 4.2% of carbohydrates, half of which is represented by glucose. The lectin reacts equally with N-acetyl and N-glycolyl neuraminic acid, free, in alpha2,3- or alpha2,6-linkage. TFL has 7-fold weaker affinity to alpha2,8-linked N-acetylneuraminic acid (Neu5Ac) in colominic acid. Horse erythrocytes containing 4-O-acetyl Neu5Ac are agglutinated equally as compared to the human cells. TFL affinity to 9-O-acetyl Neu5Ac is 4-fold weaker as documented by hemagglutination inhibition with de-O-acetylated bovine submaxillary mucin, and ovine submaxillary mucin. A panel of mono- and oligosaccharides other than Neu5Ac do not inhibit TFL activity at 200 mM. The lectin does not require bivalent cations for activity, shows optimal reactivity at neutral pH and is stable at 4 degrees C. Anti-TFL antibodies identify membrane positivity on T. foetus, suggesting that the lectin functions in adhesion of the parasites. These findings, together with good stability and immunogenicity, make TFL a prospective candidate for further studies, especially in searching for efficient diagnostics and prevention of bovine trichomoniasis. |
| | |
Authors:
|
P Babál; F F Pindak; L C Russell; W A Gardner |
Related Documents
:
|
849935 - Action of intrinsic sialidase of rat brain synaptic membranes on membrane sialolipid an... 8164395 - The relationship between the sialic acid concentrations in the serum and whole saliva i... 10026155 - Novel proteoglycan linkage tetrasaccharides of human urinary soluble thrombomodulin, so... 15229395 - Ganglioside/glycosphingolipid turnover: new concepts. 6415265 - Drug interactions i: detection of inorganic nitrite in organic nitrate esters under aci... 22280475 - Dimers of the higher-energy conformer of formic acid: experimental observation. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1428 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1999 Jun |
Date Detail:
|
Created Date: 1999-07-29 Completed Date: 1999-07-29 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 106-16 Citation Subset: IM |
Affiliation:
|
Department of Pathology, University of South Alabama, 2451 Fillingim Street, Mobile, AL 36617, USA. pbalal@jaguar1.usouthal.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis Animals Carbohydrates / analysis Cattle Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Erythrocytes / immunology Hemagglutination Tests Humans Immunohistochemistry Kinetics Lectins / chemistry, immunology, isolation & purification* Neuraminidase / chemistry* Tritrichomonas foetus / chemistry*, enzymology |
| Grant Support | |
ID/Acronym/Agency:
|
2-P41-RR05351-06/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Carbohydrates; 0/Lectins; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Single micro electrode dielectrophoretic tweezers for manipulation of suspended cells and particles.
Next Document: Urocanic acid isomers are good hydroxyl radical scavengers: a comparative study with structural anal...