Document Detail

Sialic acid recognition is a key determinant of influenza A virus tropism in murine trachea epithelial cell cultures.
MedLine Citation:
PMID:  19195676     Owner:  NLM     Status:  MEDLINE    
Influenza A virus interacts with specific types of sialic acid during attachment and entry into susceptible cells. The precise amino acids in the hemagglutinin protein that control sialic acid binding specificity and affinity vary among antigenic subtypes. For H3 subtypes, amino acids 226 and 228 are critical for differentiating between alpha2,3- and alpha2,6-linked forms of sialic acid (SA). We demonstrate that position 190 of the HA from A/Udorn/307/72 (H3N2) plays an important role in the recognition of alpha2,3-SA, as changing the residue from a glutamic acid to an aspartic acid led to alteration of red blood cell hemagglutination and a complete loss of replication in differentiated, murine trachea epithelial cell cultures which express only alpha2,3-SA. This amino acid change had a minimal effect on virus replication in MDCK cells, suggesting subtle changes in receptor recognition by the H3 hemagglutinin can lead to significant alterations in cell and species tropism.
Andrew Pekosz; Celeste Newby; Pulkit S Bose; Andrew Lutz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-02-04
Journal Detail:
Title:  Virology     Volume:  386     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-03-30     Revised Date:  2014-09-01    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  61-7     Citation Subset:  IM    
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MeSH Terms
Amino Acid Substitution / genetics
Cell Line
Cells, Cultured
Epithelial Cells / virology*
Glutamic Acid / genetics
Hemagglutinins, Viral / genetics,  metabolism
Influenza A Virus, H3N2 Subtype / genetics,  physiology*
Mice, Inbred BALB C
Mutation, Missense
N-Acetylneuraminic Acid / metabolism*
Trachea / virology
Viral Plaque Assay
Virus Attachment*
Virus Replication
Grant Support
Reg. No./Substance:
0/Hemagglutinins, Viral; 0/hemagglutinin fusogenic peptide, influenza virus; 3KX376GY7L/Glutamic Acid; GZP2782OP0/N-Acetylneuraminic Acid

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