| Sialic acid recognition is a key determinant of influenza A virus tropism in murine trachea epithelial cell cultures. | |
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MedLine Citation:
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PMID: 19195676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Influenza A virus interacts with specific types of sialic acid during attachment and entry into susceptible cells. The precise amino acids in the hemagglutinin protein that control sialic acid binding specificity and affinity vary among antigenic subtypes. For H3 subtypes, amino acids 226 and 228 are critical for differentiating between alpha2,3- and alpha2,6-linked forms of sialic acid (SA). We demonstrate that position 190 of the HA from A/Udorn/307/72 (H3N2) plays an important role in the recognition of alpha2,3-SA, as changing the residue from a glutamic acid to an aspartic acid led to alteration of red blood cell hemagglutination and a complete loss of replication in differentiated, murine trachea epithelial cell cultures which express only alpha2,3-SA. This amino acid change had a minimal effect on virus replication in MDCK cells, suggesting subtle changes in receptor recognition by the H3 hemagglutinin can lead to significant alterations in cell and species tropism. |
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Authors:
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Andrew Pekosz; Celeste Newby; Pulkit S Bose; Andrew Lutz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-02-04 |
Journal Detail:
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Title: Virology Volume: 386 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-16 Completed Date: 2009-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 61-7 Citation Subset: IM |
Affiliation:
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Dept. of Molecular Microbiology, Washington University in St. Louis School of Medicine, 660 South Euclid Avenue, Campus Box 8230, St. Louis, MO 63110, USA. apekosz@jhsph.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution
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genetics Animals Cell Line Cells, Cultured Dogs Epithelial Cells / virology* Glutamic Acid / genetics Hemagglutination Hemagglutinins, Viral / genetics, metabolism Influenza A Virus, H3N2 Subtype / genetics, physiology* Mice Mice, Inbred BALB C Mutation, Missense N-Acetylneuraminic Acid / metabolism* Plaque Assay Trachea / virology Virus Attachment* Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI053629/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hemagglutinins, Viral; 0/hemagglutinin fusogenic peptide, influenza virus; 131-48-6/N-Acetylneuraminic Acid; 56-86-0/Glutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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