Document Detail


Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi.
MedLine Citation:
PMID:  3323904     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with D-[14C]mannose or D-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by beta-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide.
Authors:
A S Couto; A M Katzin; W Colli; R M de Lederkremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  26     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-03-14     Completed Date:  1988-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  145-53     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Affinity
Chromatography, Gel
Chromatography, Paper
Electrophoresis, Paper
Glycoproteins / analysis*
Membrane Proteins / analysis*
N-Acetylneuraminic Acid
Oligosaccharides / analysis
Sialic Acids / analysis*
Trypanosoma cruzi / analysis*
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Membrane Proteins; 0/Oligosaccharides; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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