Document Detail


Sialic acid expression in autoimmune thyroiditis.
MedLine Citation:
PMID:  12553699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autoimmune diseases of the thyroid gland are among the most frequent endocrine disorders. The present study analyzes expression patterns of sialic acids in these diseases. Three lectins specific for sialic acids were used for the histochemical analysis of surgical specimens of the thyroid gland: Tritrichomonas mobilensis lectin that stains all types of sialic acids, Maackia amurensis leukoagglutinin that stains sialic acids with alpha2,3 linkage and Sambucus nigra agglutinin that stains sialic acids with alpha2,6 linkage. In autoimmune thyroiditis, there was a significant increase in sialic acid expression in epithelial cells, especially on luminal membranes of follicular cells. The alpha2,3 linkage dominated over the alpha2,6 linkage. Lymphocytes of patients with Hashimoto thyroiditis, especially in germinal centers, showed strong expression of alpha2,6-linked sialic acids on their cell membrane. Vascular endothelium was positive in all specimens. It can be concluded, that there is a significant increase in sialic acid expression in autoimmune diseases of the thyroid gland, predominantly of sialic acids with alpha2,3 linkage, whereas the sialylation pattern of lymphocytes in Hashimoto thyroiditis was also different.
Authors:
Pavol Janega; Andrea Cerná; Ivana Kholová; Eva Brabencová; Pavel Babál
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta histochemica     Volume:  104     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2002  
Date Detail:
Created Date:  2003-01-29     Completed Date:  2003-06-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  343-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, Medical Faculty, Comenius University, Bratislava, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Glycoproteins
Goiter / metabolism,  pathology
Graves Disease / metabolism,  pathology
Humans
Immunoenzyme Techniques
N-Acetylneuraminic Acid / metabolism*
Phytohemagglutinins
Plant Lectins
Ribosome Inactivating Proteins
Thyroiditis, Autoimmune / metabolism*,  pathology
Tritrichomonas / metabolism
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Phytohemagglutinins; 0/Plant Lectins; 0/Sambucus nigra lectins; 0/leukoagglutinins, plants; 131-48-6/N-Acetylneuraminic Acid; EC 3.2.2.22/Ribosome Inactivating Proteins

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