Document Detail

Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid.
MedLine Citation:
PMID:  16190615     Owner:  NLM     Status:  MEDLINE    
The immunomodulatory activities of four sialic acid-containing milk proteins (kappa-casein, glycomacropeptide, lactoferrin, and proteose peptone-3 component) were determined, and the role of sialic acid was evaluated. Two in vitro models were used: murine splenocyte proliferation, where the effect on LPS-, Con A-, and PHA-stimulated proliferation was studied, and cytokine production in LPS-stimulated murine dendritic cells (DC). All four proteins inhibited LPS-induced splenocyte proliferation, though to different degrees, and independently of sialic acid. kappa-Casein strongly inhibited PHA-induced proliferation and had a weak inhibitory effect on Con A-induced proliferation, whereas lactoferrin stimulated Con A-induced proliferation. kappa-Casein, glycomacropeptide, and lactoferrin differentially affected cytokine production by DC: kappa-casein significantly inhibited production of TNF-alpha, IL-10, -12, -6, and -1beta, independent of sialic acid, whereas less-marked effects of glycomacropeptide and lactoferrin were seen. These findings thus point to important immunosuppressive effects of some milk proteins and indicate that they may function via different mechanisms.
Trine L Mikkelsen; Susanne Bakman; Esben S Sørensen; Vibeke Barkholt; Hanne Frøkiaer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-29     Completed Date:  2005-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7673-80     Citation Subset:  IM    
BioCentrum-DTU, Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
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MeSH Terms
Caseins / pharmacology
Cell Division / drug effects
Concanavalin A / pharmacology
Cytokines / biosynthesis
Dendritic Cells / drug effects,  immunology
Immunity / drug effects*
Lactoferrin / pharmacology
Lipopolysaccharides / pharmacology
Milk Proteins / chemistry*,  pharmacology*
N-Acetylneuraminic Acid / analysis*
Neuraminidase / pharmacology
Phytohemagglutinins / pharmacology
Spleen / cytology
Structure-Activity Relationship
Reg. No./Substance:
0/Caseins; 0/Cytokines; 0/Lactoferrin; 0/Lipopolysaccharides; 0/Milk Proteins; 0/Phytohemagglutinins; 11028-71-0/Concanavalin A; 131-48-6/N-Acetylneuraminic Acid; EC

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