Document Detail


Sialic acid concentrations in plants are in the range of inadvertent contamination.
MedLine Citation:
PMID:  16395581     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The long held but challenged view that plants do not synthesize sialic acids was re-evaluated using two different procedures to isolate putative sialic acid containing material from plant tissues and cells. The extracts were reacted with 1,2-diamino-4,5-methylene dioxybenzene and the fluorescently labelled 2-keto sugar acids analysed by reversed phase and normal phase HPLC and by HPLC-electrospray tandem mass spectrometry. No N-glycolylneuraminic acid was found in the protein fraction from Arabidopsis thaliana MM2d cells. However, we did detect 3-deoxy-D: -manno-octulosonic acid and trace amounts (3-18 pmol/g fresh weight) of a compound indistinguishable from N-acetylneuraminic acid by its retention time and its mass spectral fragmentation pattern. Thus, plant cells and tissues contain five orders of magnitude less sialic acid than mammalian tissues such as porcine liver. Similar or lower amounts of N-acetylneuraminic acid were detected in tobacco cells, mung bean sprouts, apple and banana. Yet even yeast and buffer blanks, when subjected to the same isolation procedures, apparently contained the equivalent of 5 pmol of sialic acid per gram of material. Thus, we conclude that it is not possible to demonstrate unequivocally that plants synthesize sialic acids because the amounts of these sugars detected in plant cells and tissues are so small that they may originate from extraneous contaminants.
Authors:
Reinhard Zeleny; Daniel Kolarich; Richard Strasser; Friedrich Altmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-05
Journal Detail:
Title:  Planta     Volume:  224     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-24     Completed Date:  2006-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  222-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Institute of Biochemistry, Universität für Bodenkultur Wien (BOKU), Muthgasse 18, 1190, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / metabolism*
Cattle
Chromatography, High Pressure Liquid
Liver / chemistry
Mass Spectrometry
N-Acetylneuraminic Acid / analysis*,  isolation & purification
Neuraminic Acids / analysis*
Phenylenediamines / analysis
Plant Extracts / chemistry
Sugar Acids / analysis*,  isolation & purification
Swine / metabolism
Chemical
Reg. No./Substance:
0/Neuraminic Acids; 0/Phenylenediamines; 0/Plant Extracts; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 38608-07-0/1,2-diamino-4,5-methylenedioxybenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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