Document Detail

Sialic Acid metabolism and systemic pasteurellosis.
MedLine Citation:
PMID:  15731025     Owner:  NLM     Status:  MEDLINE    
Pasteurella multocida subsp. multocida is a commensal and opportunistic pathogen of food animals, wildlife, and pets and a zoonotic cause of human infection arising from contacts with these animals. Here, an investigation of multiple serotype A strains demonstrated the occurrence of membrane sialyltransferase. Although P. multocida lacks the genes for the two earliest steps in de novo sialic acid synthesis, adding sialic acid to the growth medium resulted in uptake, activation, and subsequent transfer of sialic acid to a membrane acceptor resembling lipooligosaccharide. Two candidate-activating enzymes with homology to Escherichia coli cytidine 5'-monophospho-N-acetylneuraminate synthetase were overproduced as histidine-tagged polypeptides. The synthetase encoded by pm0187 was at least 37 times more active than the pm1710 gene product, suggesting pm0187 encodes the primary sialic acid cytidylyltransferase in P. multocida. A sialate aldolase (pm1715) mutant unable to initiate dissimilation of internalized sialic acid was not attenuated in the CD-1 mouse model of systemic pasteurellosis, indicating that the nutritional function of sialate catabolism is not required for systemic disease. In contrast, the attenuation of a sialate uptake-deficient mutant supports the essential role in pathogenesis of a sialylation mechanism that is dependent on an environmental (host) supply of sialic acid. The combined results provide the first direct evidence of sialylation by a precursor scavenging mechanism in pasteurellae and of a potential tripartite ATP-independent periplasmic sialate transporter in any species.
Susan M Steenbergen; Carol A Lichtensteiger; Ruth Caughlan; Jackie Garfinkle; Troy E Fuller; Eric R Vimr
Related Documents :
1259535 - The effect of gradual mild acid hydrolysis on serologic activities of glycoproteins fro...
435265 - Carbohydrate composition of human placental n-acetylhexosaminidase a and b.
5721465 - Biosynthesis of intestinal mucins. sialic acids of sheep colonic epithelial mucin.
7535695 - The filamentous brush border glycocalyx, a mucin-like marker of enterocyte hyper-polari...
19007045 - Allometric prediction of the human pharmacokinetic parameters for naveglitazar.
6259115 - Role of nucleases in the isolation of plasmid deoxyribonucleic acid from pseudomonas ce...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  73     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-25     Completed Date:  2005-04-01     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1284-94     Citation Subset:  IM    
Laboratory of Sialobiology, Department of Pathobiology, Universityof Illinois at Urbana-Champaign, Urbana, IL 61802, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Outbred Strains
Cytidine Monophosphate N-Acetylneuraminic Acid / metabolism
Disease Models, Animal
Fructose-Bisphosphate Aldolase / genetics,  metabolism
Open Reading Frames / genetics
Pasteurella Infections / metabolism*,  microbiology
Pasteurella multocida / enzymology*,  genetics,  pathogenicity*
Sialic Acids / metabolism*
Sialyltransferases / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Sialic Acids; 3063-71-6/Cytidine Monophosphate N-Acetylneuraminic Acid; EC 2.4.99.-/Sialyltransferases; EC Aldolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Vibrio parahaemolyticus disruption of epithelial cell tight junctions occurs independently of toxin ...
Next Document:  The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to...