Document Detail


Sialic acid speciation using capillary electrophoresis: optimization of analyte derivatization and separation.
MedLine Citation:
PMID:  22901108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) was employed for rapid sialic acid speciation, facilitating the quantitative determination of N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac) on glycoproteins. Derivatization of the sialic acids with 2-aminoacridone (2-AMAC), using classical reductive amination in a nonaqueous solvent, led to the spontaneous decarboxylation of the sialic acid residues as determined by CE-LIF and offline mass spectrometric analysis. Modification of both the labeling conditions, to drive the decarboxylation reaction to completion and the CE-LIF parameters to separate the neutral species by complexation with a neutral coated capillary and borate reversed polarity, led to a robust platform for the rapid, sensitive, and quantitative speciation of sialic acids. The method can readily be used for quality control of recombinant biopharmaceuticals.
Authors:
Zoltan Szabo; Jonathan Bones; Andras Guttman; James Glick; Barry L Karger
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-29
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-03-04     Revised Date:  2013-09-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7638-42     Citation Subset:  IM    
Affiliation:
Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts 02115, United States.
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MeSH Terms
Descriptor/Qualifier:
Aminoacridines / chemistry
Animals
Cattle
Electrophoresis, Capillary*
Humans
Lasers
N-Acetylneuraminic Acid / analysis*
Neuraminic Acids / analysis
Orosomucoid / metabolism
Spectrometry, Fluorescence
Spectrometry, Mass, Electrospray Ionization
Grant Support
ID/Acronym/Agency:
GM 15847/GM/NIGMS NIH HHS; R01 GM015847/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Aminoacridines; 0/Neuraminic Acids; 0/Orosomucoid; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 27918-14-5/2-aminoacridone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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