| Sialic acid speciation using capillary electrophoresis: optimization of analyte derivatization and separation. | |
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MedLine Citation:
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PMID: 22901108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) was employed for rapid sialic acid speciation, facilitating the quantitative determination of N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac) on glycoproteins. Derivatization of the sialic acids with 2-aminoacridone (2-AMAC), using classical reductive amination in a nonaqueous solvent, led to the spontaneous decarboxylation of the sialic acid residues as determined by CE-LIF and offline mass spectrometric analysis. Modification of both the labeling conditions, to drive the decarboxylation reaction to completion and the CE-LIF parameters to separate the neutral species by complexation with a neutral coated capillary and borate reversed polarity, led to a robust platform for the rapid, sensitive, and quantitative speciation of sialic acids. The method can readily be used for quality control of recombinant biopharmaceuticals. |
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Authors:
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Zoltan Szabo; Jonathan Bones; Andras Guttman; James Glick; Barry L Karger |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-29 |
Journal Detail:
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Title: Analytical chemistry Volume: 84 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-18 Completed Date: 2013-03-04 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7638-42 Citation Subset: IM |
Affiliation:
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Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts 02115, United States. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminoacridines
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chemistry Animals Cattle Electrophoresis, Capillary* Humans Lasers N-Acetylneuraminic Acid / analysis* Neuraminic Acids / analysis Orosomucoid / metabolism Spectrometry, Fluorescence Spectrometry, Mass, Electrospray Ionization |
| Grant Support | |
ID/Acronym/Agency:
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GM 15847/GM/NIGMS NIH HHS; R01 GM015847/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aminoacridines; 0/Neuraminic Acids; 0/Orosomucoid; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 27918-14-5/2-aminoacridone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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