Document Detail


SiRNA Targeting for ICP4 Inhibits HSV-1 Replication in Retinal Pigment Epithelial Cells.
MedLine Citation:
PMID:  21883773     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Introduction:  This study sought to inhibit HSV-1 replication using small interfering RNA (siRNA) to targeting infected-cell polypeptide 4 (ICP4) genes to mediate transcription of early and late viral genes in HSV-1 lytic (productive) infection in retina epithelial cells (RPE). Methods:  After pre- or post-infecting with HSV-1, siRNAs were transfected into RPE. The antiviral effects of siRNA were evaluated by western blot, plaque-reduction assays, indirect immunofluorescence and RT-PCR. The viral titre was detected by the TCID50 method. Results:  siRNA decreased ICP4 expression in RPE that were infected with HSV-1 before or after siRNA transfection. Compared with HSV-1 infection alone or transfection with negative (control) siRNA, the viral titre and the RPE cytopathic effect were significantly decreased in RPE cells transfected with ICP4-targeting siRNA (50 and 100 nM) (p < 0.05). The siRNA effectively silenced HSV-1 ICP4 expression on both mRNA and the protein levels (p < 0.05). The inhibition of ICP4-targeting siRNA on ICP4 protein expression was also verified by Western blot in HSV-1 infected human cornea epithelial cell, human trabecular meshwork cells and Vero cells. Conclusions:  ICP4-targeting siRNA can inhibit HSV-1 replication in RPE cells, providing a foundation for development of RNAi as an antiviral therapy.
Authors:
Fang Duan; Shiming Ni; Yuhong Nie; Qiang Huang; Kaili Wu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-22
Journal Detail:
Title:  Clinical & experimental ophthalmology     Volume:  -     ISSN:  1442-9071     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100896531     Medline TA:  Clin Experiment Ophthalmol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Authors. Clinical and Experimental Ophthalmology © 2011 Royal Australian and New Zealand College of Ophthalmologists.
Affiliation:
Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, Guangzhou 510060, P.R. China Department of Ophthalmology, Renmin Hospital, Wuhan University, Wuhan 430060, P.R. China.
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