Document Detail


Should varicella-zoster virus culture be eliminated? A comparison of direct immunofluorescence antigen detection, culture, and PCR, with a historical review.
MedLine Citation:
PMID:  23035203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparison of direct fluorescent-antibody assay (DFA), culture, and two PCR assays disclosed sensitivities of 87.8%, 46.3%, and 97.6% and 100%, respectively. We reviewed 1,150 results for clinical specimens submitted for DFA and culture and found that only 17 were culture positive/DFA negative. The incremental cost to detect these 17 positives was $3,078/specimen.
Authors:
D A Wilson; B Yen-Lieberman; S Schindler; K Asamoto; J D Schold; G W Procop
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Review     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4120-2     Citation Subset:  IM    
Affiliation:
Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Clinical Laboratory Techniques / methods*
Fluorescent Antibody Technique, Direct / methods
Herpesviridae Infections / diagnosis*,  virology
Herpesvirus 3, Human / genetics,  growth & development,  immunology,  isolation & purification*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Virus Cultivation / methods
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