Document Detail

Shotgun cholanomics of ileal fluid.
MedLine Citation:
PMID:  22986022     Owner:  NLM     Status:  MEDLINE    
In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn's disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn's disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or tri-hydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.
Yingjie Chen; Michael Ogundare; Christopher M Williams; Yuchen Wang; Yuqin Wang; Gavin W Sewell; Philip J Smith; Farooq Z Rahman; Nuala O'Shea; Anthony W Segal; William J Griffiths
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-14
Journal Detail:
Title:  Biochimie     Volume:  95     ISSN:  1638-6183     ISO Abbreviation:  Biochimie     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-22     Completed Date:  2013-08-05     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  461-3     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Masson SAS. All rights reserved.
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MeSH Terms
Bile Acids and Salts / metabolism*
Body Fluids / metabolism*
Case-Control Studies
Crohn Disease / metabolism
Ileum / metabolism*
Mass Spectrometry / methods*
Grant Support
BBC5113561//Biotechnology and Biological Sciences Research Council; BBC5157712//Biotechnology and Biological Sciences Research Council; BBH0010181//Biotechnology and Biological Sciences Research Council; BBI0017351//Biotechnology and Biological Sciences Research Council; G0902005//Medical Research Council; G0902022//Medical Research Council; //Medical Research Council; //Wellcome Trust
Reg. No./Substance:
0/Bile Acids and Salts

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