Document Detail

Short term storage of samples containing monoamines: ascorbic acid and glutathione give better protection against degradation than perchloric acid.
MedLine Citation:
PMID:  9262146     Owner:  NLM     Status:  MEDLINE    
In order to study the protection of monoamines from degradation during short-time storage, the effect of three different antioxidants on the degradation of dopamine, dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) was analyzed after 5 and 20 h. The results showed that dopamine was still quite stable after 20 h storage at room temperature, but that about 95% of 5-HIAA had disappeared. The best protection against degradation of all three substances was achieved when 15% v/v of a solution containing 1-2 mM ascorbic acid or 40 mM glutathione was added to the sample, resulting in near 100% protection after 20 h. Perchloric acid actually accelerated the degradation of 5-HIAA.
F Boix; G Wøien; T Sagvolden
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  75     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-30     Completed Date:  1997-09-30     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-73     Citation Subset:  IM    
Department of Physiology, National Institute of Occupational Health, Oslo, Norway.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis
Ascorbic Acid / chemistry*
Biogenic Monoamines / analysis*,  chemistry
Chromatography, High Pressure Liquid
Dopamine / analysis
Drug Storage
Glutathione / chemistry*
Hydroxyindoleacetic Acid / analysis
Perchloric Acid / chemistry*
Reg. No./Substance:
0/Biogenic Monoamines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 50-81-7/Ascorbic Acid; 54-16-0/Hydroxyindoleacetic Acid; 70-18-8/Glutathione; 7601-90-3/Perchloric Acid

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