Document Detail

Short-term storage of cane toad (Bufo marinus) gametes.
MedLine Citation:
PMID:  11226040     Owner:  NLM     Status:  MEDLINE    
The responses of cane toad (Bufo marinus) gametes, used as a model for the development of assisted reproduction techniques for rare and endangered amphibians, to short-term storage at temperatures > 0 degrees C were studied. Whole excised testes were stored at 0 degrees or 4 degrees C for 15 days, and sperm motility was measured at excision and after storage for 2, 5, 7, 10, 12 and 15 days. Spermatozoa showed > 50% motility for 7 days at 0 degrees C and for 5 days at 4 degrees C. At 15 days, only spermatozoa stored at 0 degrees C still showed some motility (3%). Sperm suspensions were prepared at 5 day intervals over 30 days in simplified amphibian ringer (SAR) at dilutions of 1:1, 1:5 and 1:10 (w/v) testes:SAR. Aliquots from each dilution were stored at 0 degrees C in Eppendorf tubes opened at 5 day intervals of storage (aerated) or kept sealed (unaerated) (treatments: aerated or unaerated; 5, 10, 15, 20, 25 and 30 days storage). After 30 days, sperm motility and fertilizing capacity were determined. The optimal protocol for sperm storage up to 10 days, as assessed by the retention of fertilizing capacity, was as a 1:5 testis:SAR (w/v) suspension, whereas the longest absolute retention of both motility and fertilizing capacity was observed in concentrated (1:1 dilution), anaerobic suspensions (up to 25-30 days). Oviductal oocytes placed in SAR at 5, 10, 15, 20 and 25 degrees C immediately after ovulation lost viability when cooled rapidly to 5 degrees C and stored for 2 h. However, oocytes retained viability for up to 8 h at the optimum storage temperature of 15 degrees C. Thus, it is concluded that during short-term storage spermatozoa retain viability for longer than oocytes, and that spermatozoa in suspensions retain viability for longer than spermatozoa stored in situ in excised testes.
R K Browne; J Clulow; M Mahony
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  121     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-08-28     Completed Date:  2001-09-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  167-73     Citation Subset:  IM    
Department of Biological Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.
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MeSH Terms
Bufo marinus*
Cell Survival
Cold Temperature
Oocytes / physiology*
Semen Preservation
Sperm Motility
Spermatozoa / physiology*
Testis / cytology,  physiology
Time Factors
Tissue Preservation*
Reg. No./Substance:

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