| Short-term statin administration in hypercholesterolemic rabbits resistant to postconditioning: effects on infarct size, endothelial nitric oxide synthase and nitro-oxidative stress. | |
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MedLine Citation:
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PMID: 22411971 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIMS: The effectiveness of postconditioning (POC) in hypercholesterolemia is in dispute. We investigated the effects of 3-day lipophilc (simvastatin) or hydrophilic (pravastatin) statin treatment, without or with POC in normocholesterolemic (Norm) and hypercholesterolemic (Chol) rabbits. METHODS AND RESULTS: Norm or Chol rabbits were subjected to 30-min ischemia and randomized in two series of 12 groups each: Control, simvastatin (Sim), pravastatin (Prav), POC, Sim-POC, Prav-POC, Chol, Sim-Chol, Prav-Chol, POC-Chol, Sim-POC-Chol, Prav-POC-Chol. After ischemia, rabbits of the first series underwent 3-hour reperfusion, followed by infarct size, total cholesterol and LDL plasma levels evaluation, and of the second series underwent 10-min reperfusion followed by tissue sampling for nitrotyrosine (NT), malondialdehyde (MDA), endothelial nitric oxide synthase (eNOS) and Akt analyses. N-nitro-L-arginine methylester (L-NAME) was given in two additional groups (POC-L-NAME, and Prav-Chol-L-NAME) for infarct size assessment. All interventions reduced infarction in Norm (24.3±1.3%, 25.9±2.8%, 27.9±3.1%, 23.3±2.3% and 33.4±2.5%, in POC, Sim, Prav, Sim-POC and Prav-POC groups, respectively vs 49.3±1.9% in Control, p<0.05), but only Prav did so in Chol animals (25.7±3.3% and 25.3±3.9% in Prav-Chol and Prav-POC-Chol vs 50.9± 1.7%, 44.8±4.3%, 41.5±3.5% and 49.3± 5.5% in Chol, Sim-Chol, POC-Chol and Sim-POC-Chol, p<0.05).L-NAME abolished the infarct size limiting effect of POC and Prav-Chol. Prav induced the greatest reduction in NT, while it was the only intervention that increased myocardial eNOS and Akt in Chol rabbits (p<0.05 vs all others). CONCLUSION: Prav, in contrast to same-dose Sim or POC, reduces infarction in Chol rabbits independently of lipid lowering, potentially through eNOS activation and nitro-oxidative stress attenuation. |
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Authors:
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Ioanna Andreadou; Dimitrios Farmakis; Eftihios Prokovas; Fragiska Sigala; Anastasia Zoga; Katerina Spyridaki; Apostolos Papalois; Andreas Papapetropoulos; Maria Anastasiou-Nana; Dimitrios Th Kremastinos; Efstathios K Iliodromitis |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-12 |
Journal Detail:
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Title: Cardiovascular research Volume: - ISSN: 1755-3245 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Pharmaceutical Chemistry, University of Athens School of Pharmacy, Athens, Greece. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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