Document Detail

Short-term modulation of distal tubule fluid nitric oxide in vivo by loop NaCl reabsorption.
MedLine Citation:
PMID:  14675049     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Intrarenal nitric oxide (NO) production and signaling effects are influenced by NaCl loading. To gain further insight into NO mechanisms we determined whether rat distal tubular fluid (DTF) [NO] and collected NO may acutely change when NaCl loop delivery is altered. METHODS: An NO microelectrode was used to measure real-time DTF [NO] and DT-collected NO. With proximal flow blocked (open system), 150 mmol/L NaCl, with and without 10(-4) mol/L furosemide was perfused with measurement of loop [Cl] reabsorption. Using a closed system, DTF [NO] was also determined using several different loop perfusates. RESULTS: In the open system, perfusion with 40 nL/min of 150 mmol/L NaCl to which 10(-4) mol/L furosemide was added, DT [NO] and DT-collected NO was approximately twice that measured with perfusion of 150 mmol/L NaCl alone, while loop Cl reabsorption decreased by half. In the closed system, perfusion at 10 nL/min of 150 mmol/L NaCl + furosemide 10(-4) mol/L also induced a significant rise in DTF [NO] and collected NO. Perfusion of 10(-3) mol/L S-methyl-L-thiocitrulline (SMTC) with 150 mmol/L NaCl, induces a significant drop in DT [NO], but without a significant increase in collected NO. Furthermore, with addition of 10(-3) mol/L SMTC to the 150 mmol/L NaCl + 10(-4) furosemide perfusate, the rise in DT [NO] was prevented. Analysis of covariance showed that flow changes within, or between all groups, had no significant additional effect. CONCLUSION: In both open and closed loop perfusion systems, 10(-4) mol/L furosemide inhibition of NaCl transport stimulates net loop NO emission independent of flow; 10(-3) mol/L SMTC + 150 mmol/L NaCl reduces DT [NO], but not DT-collected NO. Short-term net NO emission from the entire loop, as collected in distal tubule fluid, increases with inhibition of loop NaCl transport.
David Z Levine; Kevin D Burns; James Jaffey; Michelle Iacovitti
Related Documents :
21964559 - Microbubble transport through a bifurcating vessel network with pulsatile flow.
22164449 - Microbubble generation and transmission of medtronic's affinity hardshell venous reserv...
7924759 - Effect of nimodipine on cerebral and organ blood flow in normal and posthypoxemic newbo...
1760509 - Flow rate measurements in isolated perfused kidney tubules by fluorescence photobleachi...
3618789 - Gfr regulation and flow-dependent electrophysiology of early distal tubule in amphiuma.
5338459 - Intrarenal distribution of blood flow in the transplanted dog kidney: effect of denerva...
9738619 - The distribution of infectivity in blood components and plasma derivatives in experimen...
4057699 - Flow- and pressure-adapted portal arterialization in dogs.
21641739 - Detection of ethyl glucuronide in blood spotted on different surfaces.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  65     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-16     Completed Date:  2004-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  184-9     Citation Subset:  IM    
Division of Nephrology, The Kidney Research Centre, Ottawa Health Research Institute, Ottawa, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Transport / drug effects,  physiology
Diuretics / pharmacology
Furosemide / pharmacology
Kidney Tubules, Distal / metabolism*
Nitric Oxide / metabolism*
Rats, Sprague-Dawley
Sodium Chloride / pharmacokinetics*
Reg. No./Substance:
0/Diuretics; 10102-43-9/Nitric Oxide; 54-31-9/Furosemide; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Down-regulation of Na+ transporters and AQP2 is responsible for acyclovir-induced polyuria and hypop...
Next Document:  A common sequence variation of the CLCNKB gene strongly activates ClC-Kb chloride channel activity.