Document Detail

Short-term, long-term and paracrine effect of human umbilical cord-derived stem cells in lung injury prevention and repair in experimental bronchopulmonary dysplasia.
MedLine Citation:
PMID:  23212278     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Bronchopulmonary dysplasia (BPD) remains a main complication of extreme prematurity and currently lacks efficient treatment. Rat bone marrow-derived mesenchymal stem cells (MSC) prevent lung injury in an oxygen-induced model of BPD. Human cord is an advantageous source of stem cells that is especially appealing for the treatment of neonatal diseases. The therapeutic benefit after established lung injury and long-term safety of cord-derived stem cells is unknown.
METHODS: Human cord-derived perivascular cells (PCs) or cord blood-derived MSCs were delivered prophylactically or after established alveolar injury into the airways of newborn rats exposed to hyperoxia, a well-established BPD model.
RESULTS: Rat pups exposed to hyperoxia showed the characteristic arrest in alveolar growth with air space enlargement and loss of lung capillaries. PCs and MSCs partially prevented and rescued lung function and structure. Despite therapeutic benefit, cell engraftment was low, suggesting that PCs and MSCs act via a paracrine effect. Accordingly, cell free-derived conditioned media from PCs and MSCs also exerted therapeutic benefit when used either prophylactically or therapeutically. Finally, long-term (6 months) assessment of stem cell or conditioned media therapy showed no adverse lung effects of either strategy, with persistent improvement in exercise capacity and lung structure.
CONCLUSIONS: Human umbilical cord-derived PCs and MSCs exert short- and long-term therapeutic benefit without adverse lung effects in this experimental model and offer new therapeutic options for lung diseases characterised by alveolar damage.
Maria Pierro; Lavinia Ionescu; Tiziana Montemurro; Arul Vadivel; Gaia Weissmann; Gavin Oudit; Derek Emery; Sreedhar Bodiga; Farah Eaton; Bruno Péault; Fabio Mosca; Lorenza Lazzari; Bernard Thébaud
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-04
Journal Detail:
Title:  Thorax     Volume:  68     ISSN:  1468-3296     ISO Abbreviation:  Thorax     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-15     Completed Date:  2013-06-04     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0417353     Medline TA:  Thorax     Country:  England    
Other Details:
Languages:  eng     Pagination:  475-84     Citation Subset:  IM    
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MeSH Terms
Acute Lung Injury / etiology,  physiopathology,  prevention & control*
Animals, Newborn*
Bronchopulmonary Dysplasia / complications,  pathology,  surgery*
Cells, Cultured
Culture Media, Conditioned
Disease Models, Animal
Infant, Newborn
Lung / metabolism,  pathology*
Mesenchymal Stem Cell Transplantation / methods*
Mesenchymal Stromal Cells / cytology*
Paracrine Communication
Respiratory Function Tests
Umbilical Cord / cytology*
Grant Support
G1000816//Medical Research Council; MOP 84429//Canadian Institutes of Health Research
Reg. No./Substance:
0/Culture Media, Conditioned
Comment In:
Thorax. 2013 May;68(5):402-4   [PMID:  23321604 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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