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Short-term effects of triiodothyronine on thyroid hormone receptor alpha by PI3K pathway in adipocytes, 3T3-L1.
MedLine Citation:
PMID:  25465606     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
MATERIALS AND METHODS: It was examined the involvement of PI3K pathway in mediating T3 effects by treating 3T3-L1 adipocytes with physiological (P=10nM) or supraphysiological (SI =100 nM) T3 doses during one hour (short time), in the absence or the presence of PI3K inhibitor (LY294002). The absence of any treatment was considered the control group (C). RT-qPCR was used for mRNA expression analyzes. For data analyzes ANOVA complemented with Tukey's test was used at 5% significance level. Results T3 increased TRα mRNA expression in P (1.91±0.13, p<0.001), SI (2.14±0.44, p<0.001) compared to C group (1±0.08). This increase was completely abrogated by LY294002 in P (0.53±0.03, p<0.001) and SI (0.31±0.03, p<0.001). To examine whether TRα is directly induced by T3, we used the translation inhibitor cycloheximide (CHX). The presence of CHX completely abrogated levels TRα mRNA in P (1.15±0.05, p>0.001) and SI (0.99±0.15, p>0.001), induced by T3. Conclusion These results demonstrate that the activation of the PI3K signaling pathway has a role in T3-mediated indirect TRα gene expression in 3T3-L1 adipocytes.
Authors:
Miriane de Oliveira; Regiane Marques Castro Olimpio; Maria Teresa De Sibio; Fernanda Cristina Fontes Moretto; Renata de Azevedo Mello Luvizotto; Célia Regina Nogueira
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arquivos brasileiros de endocrinologia e metabologia     Volume:  58     ISSN:  1677-9487     ISO Abbreviation:  Arq Bras Endocrinol Metabol     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403437     Medline TA:  Arq Bras Endocrinol Metabol     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  833-7     Citation Subset:  IM    
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