| Short-term effects of thyroid hormones and 3,5-diiodothyronine on membrane transport systems in chick embryo hepatocytes. | |
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MedLine Citation:
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PMID: 11956147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rapid nongenomic effects of thyroid hormones L-T(3) and L-T(4) on two plasma membrane transport systems were investigated in 14-d-old and 19-d-old chick embryo hepatocytes. The Na(+)/H(+) exchanger activity was measured using the intracellular pH-sensitive fluorescent probe 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester, whereas the amino acid transport was estimated by [1-(14)C]-2-aminoisobutyric acid uptake. System A amino acid transport activation was linear to hormone concentration, whereas the Na/H exchanger gave a bell-shaped dose-response curve, with a maximum at the physiological hormone concentration of 1 nM. The specificity of the effect was verified by the use of inhibitors and analogues. The thyroid hormone analog 3,5-diiodo-L-thyronine was able to mimic some of the hormone effects, but with a lower efficiency. The effect on the Na(+)/H(+) exchanger was identified for 14-d-old and 19-d-old cells, whereas the amino acid transport could only be activated at the late stage of embryo development. Both transport systems were activated through a signal transduction pathway involving PKC, MAPK pathway, and PI3K, even though the differences in response behavior indicate a differential modulation of the two transport systems by L-T(3) and L-T(4). These results clearly demonstrate the existence of rapid nongenomic action of thyroid hormones also in avian cells, and show that activation of System A amino acid transport is not directly correlated to changes in intracellular pH. For the first time, evidence is presented which suggests that short-term effects of thyroid hormones may play a role during fetal development and cell differentiation. |
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Authors:
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Sandra Incerpi; Paolo De Vito; Paolo Luly; Silvana Spagnuolo; Silvia Leoni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 143 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-04-16 Completed Date: 2002-05-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 1660-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Biology, University of Rome Roma Tre, Viale Marconi 446, 00146 Rome, Italy. incerpi@uniroma3.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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antagonists & inhibitors,
metabolism Amino Acids / metabolism Animals Biological Transport, Active / drug effects Cell Membrane / drug effects, metabolism Chick Embryo Diiodothyronines / pharmacology* Enzyme Inhibitors / pharmacology Hepatocytes / drug effects, metabolism* Hydrogen-Ion Concentration Mitogen-Activated Protein Kinases / antagonists & inhibitors, metabolism Protein Kinase C / antagonists & inhibitors, metabolism Signal Transduction / drug effects Sodium-Hydrogen Antiporter / metabolism Thyroid Hormones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Diiodothyronines; 0/Enzyme Inhibitors; 0/Sodium-Hydrogen Antiporter; 0/Thyroid Hormones; 534-51-0/3,5-diiodothyronine; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.13/Protein Kinase C; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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