Document Detail


Short-term dentinogenic response of dog dental pulp tissue after its induction by demineralized or native dentine, or predentine.
MedLine Citation:
PMID:  1622338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The events initiating the expression of odontoblastic potential by pulpal ectomesenchymal cells were investigated by exposing the pulp to demineralized, native and unmineralized autogenous dentine. The pulp responses to implants were histologically evaluated 3, 7 and 10 days postoperatively, while the surface structure of the newly mineralized matrices was examined 12 and 28 days after implantation. Differentiation of odontoblast-like cells in close proximity to the implanted matrix was consistently demonstrated after exposure to predentine. Scattered columnal cells undergoing polarization, characterized ultrastructurally by the orientation of their rough endoplasmic reticulum, were also found in direct contact with the demineralized dentine. However, in response to demineralized implants, groups of differentiated odontoblast-like cells were clearly seen only in association with a zone of matrix secreted in a polar, predentine-like pattern, indicating an asynchronous inductive influence of this type of implant on pulp cells. Further, the response of pulp cells to native dentine was characterized by the elaboration of a two-layered matrix (a fibrous and a polarly deposited matrix) before initiation of secondary dentinogenesis. Scanning electron microscopy of the newly deposited matrices revealed differences between the indirect matrix synthesis, observed in short-term response to implants of demineralized or native dentine, and the specific, dentinogenic function of the odontoblast-like cells. These observations indicate that the dentine-induced dentinogenesis is initiated by two mechanisms--direct induction of odontoblast-like cells as well as indirect matrix synthesis, which further controls cell polarization. Immobilization of the cells on implanted matrix seems to be the critical requirement for direct expression of the odontoblastic phenotype.
Authors:
D Tziafas; I Kolokuris; A Alvanou; K Kaidoglou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of oral biology     Volume:  37     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-08-06     Completed Date:  1992-08-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  119-28     Citation Subset:  D; IM    
Affiliation:
Department of Dental Pathology and Therapeutics, School of Dentistry, Aristotelian University of Thessaloniki, Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Nucleus / ultrastructure
Cytoplasm / ultrastructure
Dental Pulp / cytology,  physiology*,  ultrastructure
Dentin / cytology,  physiology*,  ultrastructure
Dentin, Secondary / cytology,  physiology*,  ultrastructure
Dentinogenesis / physiology*
Dogs
Ectoderm / cytology,  physiology
Extracellular Matrix / physiology,  ultrastructure
Fibroblasts / cytology,  physiology
Mesoderm / cytology,  physiology
Microscopy, Electron
Microscopy, Electron, Scanning
Odontoblasts / cytology,  physiology*,  ultrastructure
Organelles / ultrastructure

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