| Short peptide fragments derived from HMG-I/Y proteins bind specifically to the minor groove of DNA. | |
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MedLine Citation:
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PMID: 8172908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Short peptides derived from chromosomal proteins have previously been proposed to bind specifically to the minor groove of A,T-rich DNA [for a review, see M. E. A. Churchill and A. A. Travers (1991) Trends Biochem. Sci. 16, 92-97]. Using NMR spectroscopy, we investigated the DNA binding of SPRKSPRK, which is one such A,T-specific motif. Under the conditions studied SPRKSPRK interacts only nonspecifically with d(CGCAAAAAAGGC).d(GCCTTTTTTGCG). The peptides TPKRPRGRPKK, PRGRPKK, and PRGRP derived from the non-histone chromosomal protein HMG-I/Y, however, bind specifically to the central A,T sites of d(CGCAAATTTGCG)2 and d(CGCGAATTCGCG)2. 2D NOE measurements show that the RGR segment of each peptide is in contact with the minor groove. The arginine side chains and the peptide backbone are buried deep in the minor groove, in a fashion generally similar to the antibiotic netropsin. Under the same conditions the peptide PKGKP does not interact with the same oligonucleotide duplexes, indicating that the arginine guanidinium groups are major determinants of the A,T specificity. |
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Authors:
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B H Geierstanger; B F Volkman; W Kremer; D E Wemmer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 33 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-06-08 Completed Date: 1994-06-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5347-55 Citation Subset: IM |
Affiliation:
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Graduate Group in Biophysics, University of California, Berkeley 94720. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Binding Sites Carrier Proteins / chemistry*, metabolism Chromosomal Proteins, Non-Histone / chemistry*, metabolism DNA / chemistry*, metabolism Distamycins / chemistry High Mobility Group Proteins / chemistry*, metabolism Hydrogen Bonding Magnetic Resonance Spectroscopy Models, Molecular Molecular Sequence Data Netropsin / chemistry Oligodeoxyribonucleotides / chemical synthesis, chemistry*, metabolism Oligopeptides / chemical synthesis, chemistry*, metabolism Peptide Fragments / chemical synthesis, chemistry*, metabolism X-Ray Diffraction |
| Grant Support | |
ID/Acronym/Agency:
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GM-43129/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Distamycins; 0/High Mobility Group Proteins; 0/Oligodeoxyribonucleotides; 0/Oligopeptides; 0/Peptide Fragments; 1438-30-8/Netropsin; 9007-49-2/DNA |
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