Document Detail


Short-interfering RNA-mediated silencing of proliferating cell nuclear antigen inhibit proliferation and induce apoptosis in HeLa cells.
MedLine Citation:
PMID:  17466038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proliferating cell nuclear antigen (PCNA) is an important protein for DNA polymerase delta in the nucleus, and shown to have a fundamental role in cellular proliferation. It is overexpressed to support cell growth in cervical carcinoma. To study its role in stress response, we design and use short hairpin RNA (shRNA) to inhibit PCNA expression in HeLa cells and validate its effect on cell proliferation. In this study, three PCNA-shRNA expression vectors are constructed and introduced into HeLa cells, and the cell cycle is analyzed by flow cytometry. Apoptotic cell is detected by single cell gel electrophoresis assay (comet assay), and caspase cleavage is studied also. Expression of PCNA is assessed by real-time reverse transcription-polymerase chain reaction and Western blot analysis. Upon transient transfection with plasmid encoding shRNA, it is found that expression of PCNA decreased in shRNA-transfected cells, downregulation of PCNA inhibit cell growth and induce apoptosis in HeLa cells. PCNA downregulation also increase cell population in the G0-G1 phase. In conclusion, our findings demonstrate that shRNA can inhibit the DNA replication and induce apoptosis in HeLa cells effectively and, therefore, could be used as a new potential anticancer tool for therapy of human cervical carcinoma.
Authors:
H Hao; T Xin; Y Nancai; W Yanxia; L Qian; M Wei; Y Yandong; H Hanju
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Publication Detail:
Type:  Journal Article     Date:  2007-04-26
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  18     ISSN:  1525-1438     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-42     Citation Subset:  IM    
Affiliation:
Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Blotting, Western
Cell Proliferation
Comet Assay
Flow Cytometry
G0 Phase / physiology
G1 Phase / physiology
Genetic Vectors
Hela Cells
Humans
Proliferating Cell Nuclear Antigen / chemistry,  genetics*,  metabolism
RNA Interference / drug effects*
RNA, Messenger / antagonists & inhibitors,  genetics,  metabolism
RNA, Small Interfering / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Chemical
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 0/RNA, Messenger; 0/RNA, Small Interfering

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