Document Detail

Short hairpin RNA targeting c-FLIP sensitizes human cervical adenocarcinoma Hela cells to chemotherapy and radiotherapy.
MedLine Citation:
PMID:  18675507     Owner:  NLM     Status:  MEDLINE    
c-FLIP inhibits caspase-8 activation and cell apoptosis mediated by death receptors. The present study aims at determining the effects of c-FLIP targeted vector-based short hairpin RNA (shRNA) on cell growth and evaluating its modulation of responsiveness to drugs and radiotherapy in cervical adenocarcinoma Hela cells. cFLIP expression of the cells transfected with shRNA against c-FLIP was significantly down-regulated after 72 h. c-FLIP silencing markedly suppressed cell proliferation and increased cell apoptosis. The activation of caspase-8 and caspase-3 was induced with shRNA targeting cFLIP with the passage of time after transfection. Furthermore, Vector-based shRNA against c-FLIP subsequently increased the sensitivity to cisplatin, iritican and Co60 radiotherapy by about 4- to 6-folds in Hela cells. Our data suggest that vector-based shRNA effectively inhibited c-FLIP expression, enhanced the expression level of caspase-8 and caspase-3 to induce cell apoptosis, probably with the higher efficacy in combination therapies with conventional chemotherapy and radiotherapy in cervical adenocarcinoma.
Aiyue Luo; Wei Wang; Ni Sima; Yunping Lu; Jianfeng Zhou; Gang Xu; Hongtao Yu; Shixuan Wang; Ding Ma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  271     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2008-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  323-32     Citation Subset:  IM    
Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / genetics
CASP8 and FADD-Like Apoptosis Regulating Protein / genetics*
Caspase 8 / metabolism
Cell Proliferation
Flow Cytometry
Hela Cells
RNA / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Antineoplastic Agents; 0/CASP8 and FADD-Like Apoptosis Regulating Protein; 0/CFLAR protein, human; 63231-63-0/RNA; EC 3.4.22.-/Caspase 8

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