| Short communication: ketone body concentration in milk determined by Fourier transform infrared spectroscopy: value for the detection of hyperketonemia in dairy cows. | |
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MedLine Citation:
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PMID: 20630223 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to evaluate Fourier transform infrared (FTIR) spectrometry to measure milk ketone bodies to detect hyperketonemic cows and compare this method with milk fat to protein ratio to detect hyperketonemia. Plasma and milk samples were obtained weekly from calving to wk 9 postpartum from 69 high-producing dairy cows. The reference test for hyperketonemia was defined as plasma concentration of beta-hydroxybutyrate (BHBA) >or=1,200 micromol/L. The weekly prevalence of hyperketonemia during the first 9 wk of lactation was, on average, 7.1%. Both BHBA and acetone in milk, determined by FTIR, had a higher sensitivity (80%) to detect hyperketonemia compared with milk fat to protein ratio (66%). Specificity was similar for the 3 diagnostic tests (71, 70, and 71%). In conclusion, FTIR predictions of BHBA or acetone in milk can detect cows with hyperketonemia in early lactation with a higher accuracy compared with the use of milk fat to protein ratio. Because of the high proportion of false-positive tests, there are concerns about the practical applicability of FTIR predictions of acetone, BHBA, and fat to protein ratio in milk to detect hyperketonemic cows. |
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Authors:
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A T M van Knegsel; S G A van der Drift; M Horneman; A P W de Roos; B Kemp; E A M Graat |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 3065-9 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands. Ariette.vanKnegsel@wur.nl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cattle Diseases / diagnosis* Dairying / methods* Fats / analysis Ketones / analysis* Ketosis / diagnosis, veterinary* Milk / chemistry* Milk Proteins / analysis Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared* |
| Chemical | |
Reg. No./Substance:
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0/Fats; 0/Ketones; 0/Milk Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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