Document Detail

Short coincubation of gametes in in vitro fertilization improves implantation and pregnancy rates: a prospective, randomized, controlled study.
MedLine Citation:
PMID:  14556826     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine the benefits of short coincubation of gametes compared to prolonged incubation in in vitro fertilization (IVF). DESIGN: Prospective randomized controlled study. SETTING: Hospital-based fertility center. PATIENT(S): One hundred thirty patients (group 1) and 129 patients (group 2). INTERVENTION(S): Oocytes from group 1 were exposed to spermatozoa for 2 hours, and oocytes from group 2 were exposed to spermatozoa for 20 hours. MAIN OUTCOME MEASURE(S): Fertilization and cleavage rates, embryo quality, and pregnancy and implantation rates were evaluated. Estradiol (E(2)) and progesterone (P(4)) levels were measured in the wells of culture dishes after 2-hour exposure of oocytes/zygotes to spermatozoa in group 1 and after 20-hour exposure in both the groups. RESULT(S): There was no difference between the two groups in the fertilization rate and the number of embryos obtained. However, the proportion of grade 1 embryos was significantly higher among group 1 compared to group 2 patients. Clinical pregnancy and implantation rates were significantly higher among group 1 compared to group 2. The significantly higher levels of E(2) and P(4) in the 20-hour cultures compared with the 2-hour cultures may have been detrimental to embryo quality, pregnancy, and implantation rates. CONCLUSION(S): Coincubation of gametes for 2 hours with standard or high insemination concentrations of spermatozoa significantly improved embryo quality and the pregnancy and implantation rates compared with overnight incubation of gametes.
Suresh Kattera; Christopher Chen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Fertility and sterility     Volume:  80     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-14     Completed Date:  2003-11-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1017-21     Citation Subset:  IM    
Centre for Reproductive Medicine and Gleneagles IVF Centre, Gleneagles Hospital, Singapore.
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MeSH Terms
Coculture Techniques
Embryo Implantation*
Estradiol / metabolism
Fertilization in Vitro*
Infertility / therapy*
Oocytes* / metabolism
Pregnancy Rate*
Progesterone / metabolism
Spermatozoa* / metabolism
Time Factors
Reg. No./Substance:
50-28-2/Estradiol; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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