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Short-Term Effect of Cryotherapy on Human Scleral Tissue by Atomic Force Microscopy.
MedLine Citation:
PMID:  23225601     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This study investigated the inflammatory effect of cryotherapy application on collagen matrix network in human infant sclera. Donor scleral tissues taken from three infant patients divided into five groups: control group, sham-treated group, and three cryotreated groups. In the cryotherapy groups, the sclera was treated for 5 s, 10 s, and 20 s with -80°C freezing by a cryosurgical system. The cryotreated reactions were examined using double histological analysis with hematoxylin-eosin and Masson's trichrome, and atomic force microscopy analysis to quantify the diameter and D-banding of collagen fibrils. The infant scleral tissues treated with cryotherapy showed a significantly increased collagen density associated with inflammatory response (p < 0.05), increased fibril diameter (p < 0.005) compared to the scleral tissues in the control group. The results directly suggest that the cryotherapy affects the morphology of scleral collagen. SCANNING 00: 1-6, 2012. © Wiley Periodicals, Inc.
Authors:
Seung Jun Lee; Samjin Choi; Moo Sang Kim; Youjin Cheong; Hyung-Woo Kwak; Hun-Kuk Park; Kyung-Hyun Jin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Scanning     Volume:  -     ISSN:  1932-8745     ISO Abbreviation:  Scanning     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903371     Medline TA:  Scanning     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© Wiley Periodicals, Inc.
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea.
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