Document Detail


Short ligands affect modes of QD uptake and elimination in human cells.
MedLine Citation:
PMID:  21612298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to better understand nanoparticle uptake and elimination mechanisms, we designed a controlled set of small, highly fluorescent quantum dots (QDs) with nearly identical hydrodynamic size (8-10 nm) but with varied short ligand surface functionalization. The properties of functionalized QDs and their modes of uptake and elimination were investigated systematically by asymmetrical flow field-flow fractionation (AF4), confocal fluorescence microscopy, flow cytometry (FACS), and flame atomic absorption (FAA). Using specific inhibitors of cellular uptake and elimination machinery in human embryonic kidney cells (Hek 293) and human hepatocellular carcinoma cells (Hep G2), we showed that QDs of the same size but with different surface properties were predominantly taken up through lipid raft-mediated endocytosis, however, to significantly different extents. The latter observation infers the contribution of additional modes of QD internalization, which include X-AG cysteine transporter for cysteine-functionalized QDs (QD-CYS). We also investigated putative modes of QD elimination and established the contribution of P-glycoprotein (P-gp) transporter in QD efflux. Results from these studies show a strong dependence between the properties of QD-associated small ligands and modes of uptake/elimination in human cells.
Authors:
Noura A Al-Hajaj; Alexandre Moquin; Kevin D Neibert; Ghareb M Soliman; Françoise M Winnik; Dusica Maysinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-03
Journal Detail:
Title:  ACS nano     Volume:  5     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-28     Completed Date:  2011-10-26     Revised Date:  2011-11-30    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4909-18     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir-William-Osler, Room 1314, McIntyre Medical Sciences Building, Montreal, QC, H3G 1Y6, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / methods
Cell Line
Cell Separation
Flow Cytometry
HEK293 Cells
Hep G2 Cells
Humans
Ligands*
Microscopy, Confocal / methods
Nanostructures / chemistry
Nanotechnology / methods
P-Glycoprotein / metabolism
Quantum Dots*
Spectrophotometry, Atomic / methods
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Ligands; 0/P-Glycoprotein
Comments/Corrections
Comment In:
ACS Nano. 2011 Oct 25;5(10):7690; author reply 7691-2   [PMID:  22023400 ]

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