Document Detail


Shining light on the microbial world the application of Raman microspectroscopy.
MedLine Citation:
PMID:  20359457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Raman microspectroscopy is a noninvasive, label-free, and single-cell technology for biochemical analysis of individual mammalian cells, organelles, bacteria, viruses, and nanoparticles. Chemical information derived from a Raman spectrum provides comprehensive and intrinsic information (e.g., nucleic acids, protein, carbohydrates, and lipids) of single cells without the need of any external labeling. A Raman spectrum functions as a molecular "fingerprint" of single cells, which enables the differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman microspectroscopy combined with stable isotope probing, fluorescent in situ hybridization, and optical tweezers offers a culture-independent approach to study the functions and physiology of unculturable microorganisms in the ecosystem. Here, we review the application of Raman microspectroscopy to microbiology research with particular emphasis on single bacterial cells.
Authors:
Wei E Huang; Mengqiu Li; Roger M Jarvis; Royston Goodacre; Steven A Banwart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-03-06
Journal Detail:
Title:  Advances in applied microbiology     Volume:  70     ISSN:  0065-2164     ISO Abbreviation:  Adv. Appl. Microbiol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-02     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370413     Medline TA:  Adv Appl Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-86     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Civil and Structural Engineering, Kroto Research Institute, University of Sheffield, Sheffield, United Kingdom. w.huang@shef.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Bacteria / chemistry*,  metabolism,  ultrastructure
Bacteriological Techniques
Carbohydrates / chemistry
Environmental Microbiology
Lipids / chemistry
Nucleic Acids / chemistry
Proteins / chemistry
Species Specificity
Spectrum Analysis, Raman*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Lipids; 0/Nucleic Acids; 0/Proteins

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