Document Detail

Shining light on the microbial world the application of Raman microspectroscopy.
MedLine Citation:
PMID:  20359457     Owner:  NLM     Status:  MEDLINE    
Raman microspectroscopy is a noninvasive, label-free, and single-cell technology for biochemical analysis of individual mammalian cells, organelles, bacteria, viruses, and nanoparticles. Chemical information derived from a Raman spectrum provides comprehensive and intrinsic information (e.g., nucleic acids, protein, carbohydrates, and lipids) of single cells without the need of any external labeling. A Raman spectrum functions as a molecular "fingerprint" of single cells, which enables the differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman microspectroscopy combined with stable isotope probing, fluorescent in situ hybridization, and optical tweezers offers a culture-independent approach to study the functions and physiology of unculturable microorganisms in the ecosystem. Here, we review the application of Raman microspectroscopy to microbiology research with particular emphasis on single bacterial cells.
Wei E Huang; Mengqiu Li; Roger M Jarvis; Royston Goodacre; Steven A Banwart
Related Documents :
35567 - Intracellular ph-determination by fluorescence measurements.
6185287 - Flow cytometric analysis of factors which influence the brdurd-hoechst quenching effect...
16643017 - High-throughput single-cell analysis for enzyme activity without cytolysis.
3479647 - Expression of a putative tumor-associated surface antigen on normal versus marek's dise...
3430637 - Characterization of isolated ventricular myocytes: two levels of resting potential.
22447857 - The wnt inhibitor dickkopf 1 and bone morphogenetic protein 4 rescue adipogenesis in hy...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-03-06
Journal Detail:
Title:  Advances in applied microbiology     Volume:  70     ISSN:  0065-2164     ISO Abbreviation:  Adv. Appl. Microbiol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-02     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370413     Medline TA:  Adv Appl Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-86     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Civil and Structural Engineering, Kroto Research Institute, University of Sheffield, Sheffield, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteria / chemistry*,  metabolism,  ultrastructure
Bacteriological Techniques
Carbohydrates / chemistry
Environmental Microbiology
Lipids / chemistry
Nucleic Acids / chemistry
Proteins / chemistry
Species Specificity
Spectrum Analysis, Raman*
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Carbohydrates; 0/Lipids; 0/Nucleic Acids; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Surface and Adhesion Properties of Lactobacilli.
Next Document:  Detection of Invasive Aspergillosis.