| Shikonin inhibits maturation of bone marrow-derived dendritic cells and suppresses allergic airway inflammation in a murine model of asthma. | |
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MedLine Citation:
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PMID: 20735407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: Shikonin exhibits a wide range of anti-inflammatory actions. Here, we assessed its effects on maturation of murine bone marrow-derived dendritic cells (BM-DCs) and on allergic reactions in a murine model of asthma. EXPERIMENTAL APPROACH: Cultured murine BM-DCs were used to investigate the effects of shikonin on expression of cell surface markers and their stimulation of T-cell proliferation and cytokine production. The therapeutic potential of shikonin was evaluated in a model of allergic airway disease. KEY RESULTS: Shikonin dose-dependently inhibited expression of major histocompatibility complex class II, CD80, CD86, CCR7 and OX40L on BM-DCs, induced by a mixture of ovalbumin (OVA; 100µg·mL(-1) ) and thymic stromal lymphopoietin (TSLP; 20ng·mL(-1) ). Shikonin-treated BM-DCs were poor stimulators of CD4(+) T lymphocyte and induced lower levels of interleukin (IL)-4, IL-5, IL-13 and tumour necrosis factor (TNF)-α release by responding T-cells. After intratracheal instillation of shikonin in OVA-immunized mice, OVA challenge induced lower IL-4, IL-5, IL-13, TNF-α and eotaxin release in bronchial alveolar lavage fluid, lower IL-4 and IL-5 production in lung cells and mediastinal lymph node cells and attenuated OVA-induced lung eosinophilia and airway hyperresponsiveness. CONCLUSION AND IMPLICATIONS: Shikonin effectively suppressed OVA + TSLP-induced BM-DC maturation in vitro and inhibited allergic inflammation and airway hyperresponsiveness in a murine model of asthma, showing good potential as a treatment for allergic asthma. Also, our model provides a novel platform for screening drugs for allergic diseases. |
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Authors:
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Chen-Chen Lee; Chien-Neng Wang; Yu-Ting Lai; Jaw-Jou Kang; Jiunn-Wang Liao; Bor-Luen Chiang; Hui-Chen Chen; Yu-Wen Cheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of pharmacology Volume: 161 ISSN: 1476-5381 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2011-05-18 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 1496-511 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, School of Medicine, China Medical University, Taichung, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Asthma / drug therapy*, immunology, pathology Bone Marrow Cells / drug effects*, immunology Bronchoalveolar Lavage Fluid Cytokines / metabolism Dendritic Cells / drug effects*, immunology Drugs, Chinese Herbal / metabolism, pharmacology Female Inflammation / drug therapy, immunology, pathology Interleukin-13 / metabolism Interleukin-4 / metabolism Interleukin-5 / metabolism Lung / immunology, pathology Mice Mice, Inbred BALB C Naphthoquinones / pharmacology* Ovalbumin / immunology Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Cytokines; 0/Drugs, Chinese Herbal; 0/Interleukin-13; 0/Interleukin-5; 0/Naphthoquinones; 0/Tumor Necrosis Factor-alpha; 0/thymic stromal lymphopoietin; 207137-56-2/Interleukin-4; 54952-43-1/shikonin; 9006-59-1/Ovalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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