| Shigella enterotoxin-2 is a type III effector that participates in Shigella-induced interleukin 8 secretion by epithelial cells. | |
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MedLine Citation:
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PMID: 21219446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously described a protein termed Shigella enterotoxin 2 (ShET-2), which induces rises in short-circuit current in rabbit ileum mounted in the Ussing chamber. Published reports have postulated that ShET-2 may be secreted by the Shigella type III secretion system (T3SS). In this study, we show that ShET-2 secretion into the extracellular space requires the T3SS in Shigella flexneri 2a strain 2457T and a ShET-2-TEM fusion was translocated into epithelial cells in a T3SS-dependent manner. The ShET-2 gene, sen, is encoded downstream of the ospC1 gene of S. flexneri, and we show that sen is cotranscribed with this T3SS-secreted product. Considering that T3SS effectors have diverse roles in Shigella infection and that vaccine constructs lacking ShET-2 are attenuated in volunteers, we asked whether ShET-2 has a function other than its enterotoxic activity. We constructed a ShET-2 mutant in 2457T and tested its effect on epithelial cell invasion, plaque formation, guinea pig keratoconjunctivitis and interleukin 8 (IL-8) secretion from infected monolayers. Although other phenotypes were not different compared with the wild-type parent, we found that HEp-2 and T84 cells infected with the ShET-2 mutant exhibited significantly reduced IL-8 secretion into the basolateral compartment, suggesting that ShET-2 might participate in the Shigella-induced inflammation of epithelial cells. |
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Authors:
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Mauricio J Farfán; Cecilia S Toro; Eileen M Barry; James P Nataro |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-02 |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 61 ISSN: 1574-695X ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-11 Completed Date: 2011-06-20 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 332-9 Citation Subset: IM |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Centro de Estudios Moleculares, Departamento de Pediatría, Hospital Luis Calvo Mackennna, Facultad de Medicina, Universidad de Chile, Santiago, Chile. mfarfan@med.uchile.cl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Enterotoxins / metabolism*, toxicity* Epithelial Cells / immunology, microbiology* Humans Interleukin-8 / immunology*, secretion* Protein Transport Shigella flexneri / immunology*, pathogenicity* |
| Grant Support | |
ID/Acronym/Agency:
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AI033096/AI/NIAID NIH HHS; AI059223/AI/NIAID NIH HHS; R01 AI033096-09/AI/NIAID NIH HHS; R01 AI059223/AI/NIAID NIH HHS; R01 AI059223-01A2/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enterotoxins; 0/Interleukin-8 |
| Comments/Corrections | |
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