Document Detail


Shigella enterotoxin-2 is a type III effector that participates in Shigella-induced interleukin 8 secretion by epithelial cells.
MedLine Citation:
PMID:  21219446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously described a protein termed Shigella enterotoxin 2 (ShET-2), which induces rises in short-circuit current in rabbit ileum mounted in the Ussing chamber. Published reports have postulated that ShET-2 may be secreted by the Shigella type III secretion system (T3SS). In this study, we show that ShET-2 secretion into the extracellular space requires the T3SS in Shigella flexneri 2a strain 2457T and a ShET-2-TEM fusion was translocated into epithelial cells in a T3SS-dependent manner. The ShET-2 gene, sen, is encoded downstream of the ospC1 gene of S. flexneri, and we show that sen is cotranscribed with this T3SS-secreted product. Considering that T3SS effectors have diverse roles in Shigella infection and that vaccine constructs lacking ShET-2 are attenuated in volunteers, we asked whether ShET-2 has a function other than its enterotoxic activity. We constructed a ShET-2 mutant in 2457T and tested its effect on epithelial cell invasion, plaque formation, guinea pig keratoconjunctivitis and interleukin 8 (IL-8) secretion from infected monolayers. Although other phenotypes were not different compared with the wild-type parent, we found that HEp-2 and T84 cells infected with the ShET-2 mutant exhibited significantly reduced IL-8 secretion into the basolateral compartment, suggesting that ShET-2 might participate in the Shigella-induced inflammation of epithelial cells.
Authors:
Mauricio J Farfán; Cecilia S Toro; Eileen M Barry; James P Nataro
Related Documents :
16870366 - Cytotoxic effect induced by retinoic acid loaded into galactosyl-sphingosine containing...
1310596 - Effects of insulin and guanosine 5'-[gamma-thio]triphosphate on fatty acid synthesis an...
18966646 - Conductometric titration of thiosulfate using new-type conductivity cells.
16052356 - Evidence of nuclear reactions in the pd lattice.
21287686 - Multi-dimensional fluorescence microscopy of living cells.
11710476 - Ongoing cell death and immune influences on regeneration in the vestibular sensory organs.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-02
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  61     ISSN:  1574-695X     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-11     Completed Date:  2011-06-20     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  332-9     Citation Subset:  IM    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Centro de Estudios Moleculares, Departamento de Pediatría, Hospital Luis Calvo Mackennna, Facultad de Medicina, Universidad de Chile, Santiago, Chile. mfarfan@med.uchile.cl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Enterotoxins / metabolism*,  toxicity*
Epithelial Cells / immunology,  microbiology*
Humans
Interleukin-8 / immunology*,  secretion*
Protein Transport
Shigella flexneri / immunology*,  pathogenicity*
Grant Support
ID/Acronym/Agency:
AI033096/AI/NIAID NIH HHS; AI059223/AI/NIAID NIH HHS; R01 AI033096-09/AI/NIAID NIH HHS; R01 AI059223/AI/NIAID NIH HHS; R01 AI059223-01A2/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/Interleukin-8
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Voiding dynamics in women with stress urinary incontinence and high-stage cystocele.
Next Document:  HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cel...