| Shifts in methanogen community structure and function associated with long-term manipulation of sulfate and salinity in a hypersaline microbial mat. | |
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MedLine Citation:
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PMID: 18177370 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methanogenesis was characterized in hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico both in situ and after long-term manipulation in a greenhouse environment. Substrate addition experiments indicate methanogenesis to occur primarily through the catabolic demethylation of non-competitive substrates, under field conditions. However, evidence for the coexistence of other metabolic guilds of methanogens was obtained during a previous manipulation of sulfate concentrations. To fully characterize methanogenesis in these mats, in the absence of competition for reducing equivalents with sulfate-reducing microorganisms, we maintained microbial mats for longer than 1 year under conditions of lowered sulfate and salinity levels. The goal of this study was to assess whether observed differences in methane production during sulfate and salinity manipulation were accompanied by shifts in the composition of methanogen communities. Culture-independent techniques targeting methyl coenzyme M reductase genes (mcrA) were used to assess the dynamics of methanogen assemblages. Clone libraries from mats sampled in situ or maintained at field-like conditions in the greenhouse were exclusively composed of sequences related to methylotrophic members of the Methanosarcinales. Increases in pore water methane concentrations under conditions of low sulfate correlated with an observed increase in the abundance of putatively hydrogenotrophic mcrA, related to Methanomicrobiales. Geochemical and molecular data provide evidence of a significant shift in the metabolic pathway of methanogenesis from a methylotroph-dominated system in high-sulfate environments to a mixed community of methylotrophic and hydrogenotrophic methanogens under low sulfate conditions. |
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Authors:
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Jason M Smith; Stefan J Green; Cheryl A Kelley; Leslie Prufert-Bebout; Brad M Bebout |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-12-19 |
Journal Detail:
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Title: Environmental microbiology Volume: 10 ISSN: 1462-2920 ISO Abbreviation: Environ. Microbiol. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-17 Completed Date: 2008-02-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883692 Medline TA: Environ Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 386-94 Citation Subset: IM |
Affiliation:
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Exobiology Branch, NASA Ames Research Center, Moffett Field, CA, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EF202615; EF202616; EF202617; EF202618; EF202619; EF202620; EF202621; EF202622; EF202623; EF202624; EF202625; EF202626; EF202627; EF202628; EF202629; EF202630; EF202631; EF202632; EF202633; EF202634; EF202635; EF202636; EF202637; EF202638; EF202639; EF202640; EF202641; EF202642; EF202643; EF202644; EF202645; EF202646; EF202647; EF202648; EF202649; EF202650; EF202651; EF202652; EF202653; EF202654; EF202655; EF202656; EF202657; EF202658; EF202659; EF202660; EF202661; EF202662; EF202663; EF202664; EF202665; EF202666; EF202667; EF202668; EF202669; EF202670; EF202671; EF202672; EF202673; EF202674; EF202675; EF202676; EF202677; EF202678; EF202679; EF202680; EF202681; EF202682; EF202683; EF202684; EF202685; EF202686; EF202687; EF202688; EF202689; EF202690; EF202691; EF202692; EF202693; EF202694; EF202695; EF202696; EF202697; EF202698; EF202699; EF202700; EF202701; EF202702; EF202703; EF202704; EF202705; EF202706; EF202707; EF202708; EF202709; EF202710; EF202711; EF202712; EF202713; EF202714; EF202715; EF202716; EF202717; EF202718; EF202719; EF202720; EF202721; EF202722; EF202723; EF202724; EF202725; EF202726; EF202727; EF202728; EF202729; EF202730; EF202731; EF202732; EF202733; EF202734; EF202735; EF202736; EF202737; EF202738; EF202739; EF202740; EF202741; EF202742; EF202743; EF202744; EF202745; EF202746; EF202747; EF202748; EF202749; EF202750; EF202751; EF202752; EF202753; EF202754; EF202755; EF202756; EF202757; EF202758; EF202759; EF202760; EF202761; EF202762; EF202763; EF202764; EF202765; EF202766; EF202767; EF202768; EF202769; EF202770; EF202771; EF202772; EF202773; EF202774; EF202775; EF202776; EF202777; EF202778; EF202779; EF202780; EF202781; EF202782; EF202783; EF202784; EF202785; EF202786; EF202787; EF202788; EF202789; EF202790; EF202791; EF202792; EF202793; EF202794; EF202795; EF202796; EF202797; EF202798; EF202799; EF202800; EF202801; EF202802; EF202803; EF202804; EF202805; EF202806; EF202807; EF202808; EF202809; EF202810; EF202811; EF202812; EF202813 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cloning, Molecular Ecosystem* Geologic Sediments / microbiology Methane / metabolism* Methanomicrobiales / classification, enzymology, genetics, growth & development* Methanosarcinales / classification, enzymology, genetics, growth & development* Mexico Molecular Sequence Data Oxidoreductases / genetics* Phylogeny Seawater / microbiology Sequence Analysis, DNA Sodium Chloride / metabolism*, pharmacology Sulfates / metabolism*, pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Sulfates; 74-82-8/Methane; 7647-14-5/Sodium Chloride; EC 1.-/Oxidoreductases; EC 2.8.4.1/methyl coenzyme M reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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