Document Detail


Shift of CMV-specific CD4+ T-cells to the highly differentiated CD45RO-CD27- phenotype parallels loss of proliferative capacity and precedes progression to HIV-related CMV end-organ disease.
MedLine Citation:
PMID:  17556025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To identify factors related to progression to CMV end-organ disease, cytokine production, proliferative capacity and phenotype of CMV-specific CD4(+) T-cells were analysed longitudinally. Numbers of IFNgamma(+)CD4(+) and IFNgamma(+)IL-2(+)CD4(+) T-cells tended to decrease in individuals progressing to AIDS with CMV end-organ disease (AIDS-CMV), whereas they remained detectable in long-term asymptomatics (LTAs) and progressors to AIDS with opportunistic infections (AIDS-OI). In parallel, CMV-specific proliferative capacity was lost in AIDS-CMV. Initially, the majority of the CMV-specific IFNgamma(+)CD4(+) T-cells were of the CD45RO(+)CD27(-) subset, but during progression to AIDS-CMV a shift in phenotype to the CD45RO(-)CD27(-) subset was observed. Our data indicate that a decrease in CMV-specific cytokine production and proliferative capacity precedes progression to AIDS-CMV. Accumulation of CD4(+) T-cells with a CD45RO(-)CD27(-) phenotype suggests that persistent antigen exposure drives differentiation of CMV-specific CD4(+) T-cells towards a poorly proliferating, and highly differentiated "effector" subset, which eventually fails to produce IFNgamma in patients developing AIDS-CMV.
Authors:
Corine Bronke; Christine A Jansen; Geertje H A Westerlaken; Iris M De Cuyper; Frank Miedema; Kiki Tesselaar; Debbie van Baarle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-06
Journal Detail:
Title:  Clinical immunology (Orlando, Fla.)     Volume:  124     ISSN:  1521-6616     ISO Abbreviation:  Clin. Immunol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-20     Completed Date:  2007-09-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100883537     Medline TA:  Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  190-9     Citation Subset:  IM    
Affiliation:
Department of Clinical Viro-Immunology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
AIDS-Related Opportunistic Infections / complications,  immunology*,  virology
Antigens, CD27 / immunology*
Antigens, CD45 / immunology*
CD4-Positive T-Lymphocytes / immunology*
Cytomegalovirus / immunology*
Cytomegalovirus Infections / immunology*,  virology
Disease Progression
HIV Infections / complications,  immunology*,  virology
HIV-1 / immunology*
Humans
Interferon-gamma / biosynthesis,  immunology
Interleukin-2 / biosynthesis,  immunology
Lymphocyte Activation
Chemical
Reg. No./Substance:
0/Antigens, CD27; 0/Interleukin-2; 82115-62-6/Interferon-gamma; EC 3.1.3.48/Antigens, CD45

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