Document Detail

Shf, a Shb-like adapter protein, is involved in PDGF-alpha-receptor regulation of apoptosis.
MedLine Citation:
PMID:  11095946     Owner:  NLM     Status:  MEDLINE    
Recent work has implicated the importance of adapter proteins in signal transduction. To identify homologues of the previously identified adapter protein Shb, database searches were performed. A Shb-like protein was found which we have named Shf. Shf contains an SH2 domain and four putative tyrosine phosphorylation sites and is mainly expressed in skeletal muscle, brain, liver, prostate, testis, ovary, small intestine, and colon. The SH2 domain of Shf bound to the PDGF-alpha-receptor at tyrosine-720, but not to the PDGF-beta-receptor in PAE cells. Pervanadate induced tyrosine phosphorylation of Shf in NIH3T3 fibroblasts overexpressing this protein, whereas PDGF-AA alone had no detectable effect. NIH3T3 cells overexpressing Shf displayed significantly lower rates of apoptosis than control cells in the presence of PDGF-AA. Our findings suggest a role for the novel adapter Shf in PDGF-receptor signaling and regulation of apoptosis.
C K Lindholm; J D Frantz; S E Shoelson; M Welsh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  278     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-12     Completed Date:  2001-01-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  537-43     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Medical Cell Biology, Uppsala University, Uppsala, S-75123, Sweden.
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MeSH Terms
3T3 Cells
Adaptor Proteins, Signal Transducing*
Amino Acid Sequence
Apoptosis / drug effects,  physiology*
Cell Division / drug effects,  physiology
Cloning, Molecular
Intracellular Signaling Peptides and Proteins
Molecular Sequence Data
Phosphoproteins / chemistry,  genetics*,  metabolism*
Phosphotyrosine / metabolism
Platelet-Derived Growth Factor / pharmacology
Proto-Oncogene Proteins / chemistry,  genetics
Receptor, Platelet-Derived Growth Factor alpha / physiology*
Recombinant Proteins / chemistry,  metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Vanadates / pharmacology
src Homology Domains
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Intracellular Signaling Peptides and Proteins; 0/Phosphoproteins; 0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins; 0/Recombinant Proteins; 0/SHB protein, human; 0/SHF protein, human; 0/Shb protein, mouse; 0/Vanadates; 0/pervanadate; 0/platelet-derived growth factor A; 21820-51-9/Phosphotyrosine; EC, Platelet-Derived Growth Factor alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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