Document Detail


Shelf-life evaluation of bilayered human skin equivalent, MyDerm™.
MedLine Citation:
PMID:  22927903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skin plays an important role in defense against infection and other harmful biological agents. Due to its fragile structure, skin can be easily damaged by heat, chemicals, traumatic injuries and diseases. An autologous bilayered human skin equivalent, MyDerm™, was engineered to provide a living skin substitute to treat critical skin loss. However, one of the disadvantages of living skin substitute is its short shelf-life, hence limiting its distribution worldwide. The aim of this study was to evaluate the shelf-life of MyDerm™ through assessment of cell morphology, cell viability, population doubling time and functional gene expression levels before transplantation. Skin samples were digested with 0.6% Collagenase Type I followed by epithelial cells dissociation with TrypLE Select. Dermal fibroblasts and keratinocytes were culture-expanded to obtain sufficient cells for MyDerm™ construction. MyDerm™ was constructed with plasma-fibrin as temporary biomaterial and evaluated at 0, 24, 48 and 72 hours after storage at 4°C for its shelf-life determination. The morphology of skin cells derived from MyDerm™ remained unchanged across storage times. Cells harvested from MyDerm™ after storage appeared in good viability (90.5%±2.7% to 94.9%±1.6%) and had short population doubling time (58.4±8.7 to 76.9±19 hours). The modest drop in cell viability and increased in population doubling time at longer storage duration did not demonstrate a significant difference. Gene expression for CK10, CK14 and COL III were also comparable between different storage times. In conclusion, MyDerm™ can be stored in basal medium at 4°C for at least 72 hours before transplantation without compromising its functionality.
Authors:
Wan Tai Seet; Maarof Manira; Manira Maarof; Khairoji Khairul Anuar; Kien-Hui Chua; Abdul Wahab Ahmad Irfan; Min Hwei Ng; Bin Saim Aminuddin; Bt Hj Idrus Ruszymah
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-23
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-28     Completed Date:  2013-01-29     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e40978     Citation Subset:  IM    
Affiliation:
Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects
Fibroblasts / cytology,  drug effects,  metabolism
Gene Expression Regulation / drug effects
Humans
Keratinocytes / cytology,  drug effects,  metabolism
Skin / cytology*
Skin Transplantation
Skin, Artificial*
Temperature
Time Factors
Comments/Corrections
Erratum In:
PLoS One. 2012;7(9). doi:10.1371/annotation/44cd1027-1f9e-4843-b013-84ca45ae942f
Note: Maarof, Manira [corrected to Manira, Maarof]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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