Document Detail

Sheet plastination of the larynx for whole-organ histology.
MedLine Citation:
PMID:  9293301     Owner:  NLM     Status:  MEDLINE    
Whole organ serial sections of the larynx are recognized to improve the assessment of laryngeal pathology. The human larynx with its complex anatomy of different types of tissue with their marked differences in hardness complicate the cutting process. The methods established are either very costly, time consuming and prone to artefacts, or sections are too thick for a histological work-up. Prior decalcification causes major shrinkage, thus morphometry is not feasible. We describe a technique of sheet plastination to produce whole-organ sections which allows a full-scale histological investigation without prior decalcification. Deep-frozen larynges are cut into 4-micron-thick sections using an ordinary slicing machine. Slices are dehydrated in acetone and plastinated in Biodur E50/E7/ AE10/E700. These specimens are cut into sections of about 80 microns thickness using a diamond-wire saw. An ultra-milling device reduces the section thickness down to about 10 microns. Surface staining of different kinds like Richardson, methylene blue or Paragon yields sufficient contrast for exact diagnosis. Deplastination and consecutive paraffin-like staining is possible, but results are not yet fully satisfying. Histological sheet plastination produces almost artefact- and shrinkage-free whole-organ sections in a period of about 1 week. The technique is useful for studies of micromorphometry or tumor spread. Theoretically, application of immunohistochemical staining methods seems to be possible. This might lead to mapping the proliferation activity of laryngeal cancer as it is presented on whole-organ sections.
C Sittel; H E Eckel; S Ricke; E Stennert
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta anatomica     Volume:  158     ISSN:  0001-5180     ISO Abbreviation:  Acta Anat (Basel)     Publication Date:  1997  
Date Detail:
Created Date:  1997-10-20     Completed Date:  1997-10-20     Revised Date:  2007-07-02    
Medline Journal Info:
Nlm Unique ID:  0370272     Medline TA:  Acta Anat (Basel)     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  74-80     Citation Subset:  IM    
Department of Otorhinolaryngology/Head and Neck Surgery, University of Cologne, Germany.
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MeSH Terms
Carcinoma / pathology,  surgery
Laryngeal Neoplasms / pathology,  surgery
Larynx / pathology*,  surgery
Microtomy / instrumentation*
Plastic Embedding*
Staining and Labeling

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