Document Detail

Shedding-induced gap formation contributes to gut barrier dysfunction in endotoxemia.
MedLine Citation:
PMID:  23271096     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The intestinal mucosa exhibits high turnover rates with a balance of shedding and the migration of epithelial cells to maintain gut barrier function. Systemic diseases such as sepsis and major thermal injury accelerate the rate of cell shedding, subsequent gap formation, and gut barrier dysfunction. However, the detailed changes of intestinal villi in barrier dysfunction have not been well described.
METHODS: In this study, intestinal barrier dysfunctions were induced through the injection of lipopolysaccharide (LPS) in C57BL/6 mice. Intravital images of the small intestine were observed with multiphoton microscopy for cellular dynamics analysis. The changes of epithelial cells shedding, gap formation, goblet cells, and intestinal leaks were observed, calculated, and analyzed.
RESULTS: Endotoxemia enhanced chromatin condensation, accelerated migration, and increased the shedding of intestinal epithelial cells compared with the control group. Furthermore, LPS-induced shedding resulted in gap formation and subsequent intestinal leaks. In total, 40% of intestinal leaks were through gaps, and 60% were through paracellular spaces. Although LPS injection significantly increased the leaks in gaps and paracellular spaces, it did not change the percentage of leaks in gaps and paracellular spaces compared with the control group.
CONCLUSION: We conclude that endotoxemia causes gut barrier dysfunction by increasing epithelium shedding, gaps, and intestinal leaks. However, the effect of the impairment of local barrier maintenance on the distribution of intestinal leaks in gaps and paracellular spaces is minimal.
Chieh-Wen Lai; Tzu-Lin Sun; Wen Lo; Zong-Han Tang; Semon Wu; Yao-Jen Chang; Chao-Chuan Wu; Sen-Chang Yu; Chen-Yuan Dong; Lee-Wei Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of trauma and acute care surgery     Volume:  74     ISSN:  2163-0763     ISO Abbreviation:  J Trauma Acute Care Surg     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-28     Completed Date:  2013-03-21     Revised Date:  2013-09-25    
Medline Journal Info:
Nlm Unique ID:  101570622     Medline TA:  J Trauma Acute Care Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  203-13     Citation Subset:  AIM; IM    
Institute of Emergency and Critical Care Medicine, National Yang-Ming University, Kaohsiung, Taiwan.
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MeSH Terms
Endotoxemia / physiopathology*
Epithelial Cells / physiology
Escherichia coli
Gap Junctions / physiology*
Intestinal Mucosa / physiopathology*
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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