Document Detail

Shedding of gangliosides from tumor cells depends on cell density.
MedLine Citation:
PMID:  6723650     Owner:  NLM     Status:  MEDLINE    
The ganglioside composition of mouse ascites hepatoma ( MAH ) cells, the ascites fluid and cell-conditioned media were determined and found to be qualitatively identical, but quantitatively different. The ganglioside content of the ascites fluid and the medium conditioned by MAH -cells at the native cell concentration (10(8) cells/ml) comprised respectively 74.9% and 23% of the cell-associated gangliosides. When incubated at lower cell-density (10(6) cells/ml) the cells were found to be release about three-times higher amounts of ganglioside per cell than during incubation at the native concentration. Centrifugation of the dense-cell-conditioned medium revealed the major part of the released gangliosides to be associated with a 150000 X g pellet that probably contains shed plasma membrane fragments. In the 150000 X g pellet of the extracellular fluids the relative content of the most polar cell ganglioside corresponding chromatographically to GT1b was about ten-times higher than in the cells. The possibility is raised that the more intense shedding of gangliosides from less crowded MAH cells may play a role in the self protection of the tumor from host immune rejection during initial stages of growth.
G I Shaposhnikova; N V Prokazova; G A Buznikov; N D Zvezdina; N A Teplitz; L D Bergelson
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  140     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-07-23     Completed Date:  1984-07-23     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  567-70     Citation Subset:  IM    
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MeSH Terms
Ascitic Fluid / metabolism
Cell Count
Gangliosides / metabolism*
Lipids / analysis
Liver Neoplasms, Experimental / metabolism*,  pathology
N-Acetylneuraminic Acid
Sialic Acids / analysis
Reg. No./Substance:
0/Gangliosides; 0/Lipids; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid

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