| Shear stress increases endothelial hyaluronan synthase 2 and hyaluronan synthesis especially in regard to an atheroprotective flow profile. | |
| | |
MedLine Citation:
|
PMID: 21551265 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Recent studies revealed that in vivo the inner blood vessel surface is lined with an endothelial surface layer of at least 0.5 μm, which serves as an aegis, protecting the vessel wall from arteriosclerosis. Hyaluronan seems to be a constitutive component in regard to the atheroprotective properties of this surface structure. It has been shown that pulsatile blood flow, found at normal vessel sites increases the size of this surface layer in vivo, while it is significantly reduced at atheroprone regions with bidirectional low flow. This study was undertaken to reveal if endothelial hyaluronan synthesis via hyaluronan synthase 2 (HAS2) can be changed by different shear stress conditions in vitro, especially in regard to an atheroprotective flow profile. Human umbilical vein endothelial cells, exposed to constant, pulsatile, and bidirectional laminar flow in a cone-and-plate system, were analysed for HAS2 expression by real-time RT-PCR and immunoblotting, and for hyaluronan by ELISA. HAS2 mRNA and protein were found to be shear stress-dependently increased via the PI3K-Akt-pathway in a transient manner. Especially atheroprotective flow induced enzyme and hyaluronan effectively, while low and bidirectional flow did not induce any differences as compared to static control cultures. These experiments provide a link between the production of a constitutive component of the endothelial surface layer by endothelial cells and different blood flow conditions. |
| | |
Authors:
|
Julian Maroski; Bernd Vorderwuelbecke; Katarzyna Fiedorowicz; Luis Da Silva-Azevedo; Guenter Siegel; Alex Marki; Axel Pries; Andreas Zakrzewicz |
Related Documents
:
|
21494795 - Diaphragm pico-liter pump for single-cell manipulation. 10968155 - Use of the uroflow study in the diagnosis of bladder outlet obstruction in elderly men. 22522535 - A microfluidic device for separating erythrocytes polluted by lead (ii) from a continuo... 8306535 - A method to evaluate renal ammoniagenesis in vivo. 17035065 - Preparation of microbubble suspensions by co-axial electrohydrodynamic atomization. 7486115 - Propofol blood concentration and the bispectral index predict suppression of learning d... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-6 |
Journal Detail:
|
Title: Experimental physiology Volume: - ISSN: 1469-445X ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9002940 Medline TA: Exp Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Charité - Campus Benjamin Franklin. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA methylation in cancer development, diagnosis and therapy--multiple opportunities for genotoxic a...
Next Document: Ion channels as target effectors for carbon monoxide.