Document Detail

Shear stress during early embryonic stem cell differentiation promotes hematopoietic and endothelial phenotypes.
MedLine Citation:
PMID:  23138937     Owner:  NLM     Status:  MEDLINE    
Pluripotent embryonic stem cells (ESCs) are a potential source for cell-based tissue engineering and regenerative medicine applications, but their translation into clinical use will require efficient and robust methods for promoting differentiation. Fluid shear stress, which can be readily incorporated into scalable bioreactors, may be one solution for promoting endothelial and hematopoietic phenotypes from ESCs. Here we applied laminar shear stress to differentiating ESCs using a 2D adherent parallel plate configuration to systematically investigate the effects of several mechanical parameters. Treatment similarly promoted endothelial and hematopoietic differentiation for shear stress magnitudes ranging from 1.5 to 15 dyne/cm(2) and for cells seeded on collagen-, fibronectin- or laminin-coated surfaces. Extension of the treatment duration consistently induced an endothelial response, but application at later stages of differentiation was less effective at promoting hematopoietic phenotypes. Furthermore, inhibition of the FLK1 protein (a VEGF receptor) neutralized the effects of shear stress, implicating the membrane protein as a critical mediator of both endothelial and hematopoietic differentiation by applied shear. Using a systematic approach, studies such as these help elucidate the mechanisms involved in force-mediated stem cell differentiation and inform scalable bioprocesses for cellular therapies.
Russell P Wolfe; Tabassum Ahsan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-02-15
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  110     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-08-05     Revised Date:  2014-06-12    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1231-42     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Bone Marrow Cells / cytology*
Cell Differentiation*
Embryonic Stem Cells / cytology*
Endothelium / cytology*
Flow Cytometry
Stress, Mechanical*
Tissue Engineering
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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