Document Detail


Shear-induced volume decrease in MDCK cells.
MedLine Citation:
PMID:  22759987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a microfluidic cell volume sensor we measured the change in the cell volume of Madin-Darby Canine Kidney (MDCK) cells induced by shear stress. An increase in shear stress from 0.2 to 2.0 dyn/cm(2) resulted in a volume decrease to a steady state volume ∼ 20 - 30 % smaller than the initial resting cell volume. Independent experiments based on fluorescence quenching confirmed the volume reduction. This shear-induced cell shrinkage was irreversible on the time scale of the experiment (∼ 30 min). Treatment of 0.1 µM Hg(2+) significantly inhibited the volume decrease, suggesting that the shear-induced cell shrinkage is associated with water efflux through aquaporins. The volume decrease cannot be inhibited by 75 mM TEA, 100 µM DIDS, or 100 µM Gd(3+) suggesting that volume reduction is not directly mediated by K(+) and Cl(-)channels that typically function during regulatory volume decrease (RVD), nor is it through cationic stretch-activated ion channels (SACs). The process also appears to be Ca(2+) independent because it was insensitive to intracellular Ca(2+) level. Since cell volume is determined by the intracellular water content, we postulate that the shear induced reductions in cell volume may arise from increased intracellular hydrostatic pressure as the cell is deformed under flow, which promotes the efflux of water. The increase in internal pressure in a deformable object under the flow is supported by the finite element mechanical model.
Authors:
Jinseok Heo; Frederick Sachs; Jianbin Wang; Susan Z Hua
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-03
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  30     ISSN:  1421-9778     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-20     Completed Date:  2012-11-20     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  395-406     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14260, USA.
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Animals
Calcium / metabolism
Cell Line
Cell Size / drug effects
Chloride Channels / metabolism
Dogs
Gadolinium / pharmacology
Mercury / pharmacology
Microfluidic Analytical Techniques
Potassium Channels / metabolism
Shear Strength*
Tetraethylammonium / pharmacology
Water / metabolism
Grant Support
ID/Acronym/Agency:
DK77302/DK/NIDDK NIH HHS; K25 DK077302/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Chloride Channels; 0/Potassium Channels; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 66-40-0/Tetraethylammonium; 7439-97-6/Mercury; 7440-54-2/Gadolinium; 7440-70-2/Calcium; 7732-18-5/Water
Comments/Corrections

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