Document Detail

Shear flow-induced formation of tubular cell protrusions in multiple myeloma cells.
MedLine Citation:
PMID:  21344380     Owner:  NLM     Status:  MEDLINE    
Exposure of live cells to shear flow induces major changes in cell shape, adhesion to the extracellular matrix, and migration. In the present study, we show that exposure of cultured multiple myeloma (MM) cells to shear flow of 4-36 dynes/cm(2) triggers the extension of long tubular protrusions (denoted flow-induced protrusions, or FLIPs) in the direction of the flow. These FLIPs were found to be rich in actin, contain few or no microtubules and, apart from endoplasmic reticulum (ER)-like membranal structures, are devoid of organelles. Studying the dynamics of this process revealed that FLIPs elongate at their tips in a shear force-dependent manner, and retract at their bases. Examination of this force dependence revealed considerable heterogeneity in the mechanosensitivity of individual cells, most likely reflecting the diversity of the malignant B cell population. The mechanisms underlying FLIP formation following mechanical perturbation, and their relevance to the cellular trafficking of MM cells, are discussed.
Ziv Porat; Itamar Yaron; Ben-Zion Katz; Zvi Kam; Benjamin Geiger
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  226     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-09-28     Completed Date:  2011-11-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3197-207     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
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MeSH Terms
Actins / metabolism
Cell Adhesion
Cell Culture Techniques
Cell Line, Tumor
Cell Movement
Cell Shape
Cell Surface Extensions / metabolism,  pathology*,  ultrastructure
Mechanotransduction, Cellular*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Microscopy, Video
Multiple Myeloma / metabolism,  pathology*,  ultrastructure
Neoplasm Invasiveness
Stress, Mechanical
Time Factors
Grant Support
PN2 EY 016586/EY/NEI NIH HHS; PN2 EY016586-06/EY/NEI NIH HHS
Reg. No./Substance:

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