| Shear flow-induced formation of tubular cell protrusions in multiple myeloma cells. | |
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MedLine Citation:
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PMID: 21344380 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exposure of live cells to shear flow induces major changes in cell shape, adhesion to the extracellular matrix, and migration. In the present study, we show that exposure of cultured multiple myeloma (MM) cells to shear flow of 4-36 dynes/cm(2) triggers the extension of long tubular protrusions (denoted flow-induced protrusions, or FLIPs) in the direction of the flow. These FLIPs were found to be rich in actin, contain few or no microtubules and, apart from endoplasmic reticulum (ER)-like membranal structures, are devoid of organelles. Studying the dynamics of this process revealed that FLIPs elongate at their tips in a shear force-dependent manner, and retract at their bases. Examination of this force dependence revealed considerable heterogeneity in the mechanosensitivity of individual cells, most likely reflecting the diversity of the malignant B cell population. The mechanisms underlying FLIP formation following mechanical perturbation, and their relevance to the cellular trafficking of MM cells, are discussed. |
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Authors:
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Ziv Porat; Itamar Yaron; Ben-Zion Katz; Zvi Kam; Benjamin Geiger |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 226 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-09-28 Completed Date: 2011-11-14 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 3197-207 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Cell Adhesion Cell Culture Techniques Cell Line, Tumor Cell Movement Cell Shape Cell Surface Extensions / metabolism, pathology*, ultrastructure Humans Mechanotransduction, Cellular* Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Microscopy, Video Multiple Myeloma / metabolism, pathology*, ultrastructure Neoplasm Invasiveness Stress, Mechanical Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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PN2 EY 016586/EY/NEI NIH HHS; PN2 EY016586-06/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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