|Shared and distinct transcriptional programs underlie the hybrid nature of iNKT cells.|
|Jump to Full Text|
|PMID: 23202270 Owner: NLM Status: MEDLINE|
|Invariant natural killer T cells (iNKT cells) are innate-like T lymphocytes that act as critical regulators of the immune response. To better characterize this population, we profiled gene expression in iNKT cells during ontogeny and in peripheral subsets as part of the Immunological Genome Project. High-resolution comparative transcriptional analyses defined developmental and subset-specific programs of gene expression by iNKT cells. In addition, we found that iNKT cells shared an extensive transcriptional program with NK cells, similar in magnitude to that shared with major histocompatibility complex (MHC)-restricted T cells. Notably, the program shared by NK cells and iNKT cells also operated constitutively in γδ T cells and in adaptive T cells after activation. Together our findings highlight a core effector program regulated distinctly in innate and adaptive lymphocytes.|
|Nadia R Cohen; Patrick J Brennan; Tal Shay; Gerald F Watts; Manfred Brigl; Joonsoo Kang; Michael B Brenner;|
|Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-12-02|
|Title: Nature immunology Volume: 14 ISSN: 1529-2916 ISO Abbreviation: Nat. Immunol. Publication Date: 2013 Jan|
|Created Date: 2012-12-14 Completed Date: 2013-03-01 Revised Date: 2014-03-07|
Medline Journal Info:
|Nlm Unique ID: 100941354 Medline TA: Nat Immunol Country: United States|
|Languages: eng Pagination: 90-9 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Genome, Human / immunology
Immunity, Innate / genetics
Immunologic Memory / genetics
Natural Killer T-Cells / immunology*
T-Lymphocyte Subsets / immunology*
Thymus Gland / growth & development, immunology*
|P30 DK063720/DK/NIDDK NIH HHS; R01 AI028973/AI/NIAID NIH HHS; R01 AI063428/AI/NIAID NIH HHS; R01AI063428/AI/NIAID NIH HHS; R24 AI072073/AI/NIAID NIH HHS; R24AI072073/AI/NIAID NIH HHS; T32AI007306/AI/NIAID NIH HHS|
|Paul Monach / ; Susan A Shinton / ; Richard R Hardy / ; Radu Jianu / ; David Koller / ; Jim Collins / ; Roi Gazit / ; Brian S Garrison / ; Derrick J Rossi / ; Kavitha Narayan / ; Katelyn Sylvia / ; Joonsoo Kang / ; Anne Fletcher / ; Kutlu Elpek / ; Angelique Bellemare-Pelletier / ; Deepali Malhotra / ; Shannon Turley / ; Adam J Best / ; Jamie Knell / ; Ananda Goldrath / ; Vladimir Jojic / ; Daphne Koller / ; Tal Shay / ; Aviv Regev / ; Nadia Cohen / ; Patrick Brennan / ; Michael Brenner / ; Taras Kreslavsky / ; Natalie A Bezman / ; Joseph C Sun / ; Charlie C Kim / ; Lewis L Lanier / ; Jennifer Miller / ; Brian Brown / ; Miriam Merad / ; Emmanuel L Gautier / ; Claudia Jakubzick / ; Gwendalyn J Randolph / ; Francis Kim / ; Tata Nageswara Rao / ; Amy Wagers / ; Tracy Heng / ; Michio Painter / ; Jeffrey Ericson / ; Scott Davis / ; Ayla Ergun / ; Michael Mingueneau / ; Diane Mathis / ; Christophe Benoist /|
Journal ID (nlm-journal-id): 100941354
Journal ID (pubmed-jr-id): 21750
Journal ID (nlm-ta): Nat Immunol
Journal ID (iso-abbrev): Nat. Immunol.
nihms-submitted publication date: Day: 16 Month: 8 Year: 2013
Electronic publication date: Day: 02 Month: 12 Year: 2012
Print publication date: Month: 1 Year: 2013
pmc-release publication date: Day: 06 Month: 9 Year: 2013
Volume: 14 Issue: 1
First Page: 90 Last Page: 99
PubMed Id: 23202270
|Shared and distinct transcriptional programs underlie the hybrid nature of iNKT cells|
|Nadia R. Cohen15|
|Patrick J. Brennan15|
|Gerald F. Watts1|
|Michael B. Brenner1|
|The Immunological Genome Project Consortium|
1Department of Medicine, Division of Rheumatology, Immunology and Allergy, Harvard Medical School, Boston, Massachusetts, USA
2Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
3Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
4Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
|Correspondence: Corresponding author: Michael B. Brenner, Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women's Hospital, Smith Building, Room 552, One Jimmy Fund Way, Boston, MA 02115, Tel: 617-525-1000, Fax: 617-525-1001, firstname.lastname@example.org
5These authors contributed equally to this work
The Immunological Genome Project (ImmGen) is a consortium of immunologists and computational biologists who aim, using rigorously standardized experimental and analysis pipelines, to generate a high-resolution, comprehensive definition of gene expression and regulatory networks in the mouse immune system1. In this context, we determined global gene expression profiles for thymic and peripheral invariant natural killer T (iNKT) cell subsets to gain insight into the iNKT cell transcriptional landscape, its unique features, and the relationships of iNKT cells to other innate and adaptive cell lineages.
iNKT cells are a subset of αβ T cells with an semi-invariant T cell antigen receptor (TCR) recognizing lipid antigens presented by CD1d2. By rapidly producing cytokines, these cells modulate both the innate and adaptive arms of the immune system, critically affecting biological processes in anti-microbial immunity, tumor rejection, and inflammation3. Like MHC-restricted T cells, iNKT cells undergo thymic differentiation with somatic recombination4, recognize self and foreign antigens2,3, secrete TH1, TH 2, and TH17 cytokines5, and provide help to B cells6.
The term “NK T” was coined to reflect the expression of the natural killer (NK) cell marker NK1.17. While a number of other NK receptors (NKRs) can also be expressed by iNKT cells8, the validity of the term “NKT” has been called into question9 because iNKT cells are developmentally more closely related to the T than the NK cell lineage, and because NKRs are neither specific to iNKT cells nor expressed on all iNKT cells.
While a central role for TCR-mediated activation in iNKT cell biology is clear, striking parallels in the behavior of both NK and iNKT cells have nevertheless emerged. The homeostatic distribution and survival requirements of iNKT cells are similar to those of NK cells10,11,12,13. Also comparable are their trafficking and activation kinetics. Both cell types constitutively express inflammatory chemokine receptors, accumulate at sites of infection within 24–72 h, and exert their effector functions without a priming requirement14,15. In addition, evidence suggests that iNKT cells, like NK cells, can use activating NKRs to sense stress-induced ligands15–19. iNKT cells also detect cellular stress via their TCRs, which are reactive to inflammation-induced alterations in CD1d-presented self-lipid antigens20–22. Finally, NK and iNKT cells engage in comparable bidirectional interactions with antigen presenting cells (APCs) during which APC-derived inflammatory cytokines potentiate NK and iNKT responses to surface ligands, and NK or iNKT cells, in return, promote APC maturation23–28.
In this report, we shed light on the transcriptional programs operating over the course of iNKT cell development and in peripheral CD4+ and CD4−iNKT cell subsets. Utilizing the ImmGen compendium, which allows direct comparison of gene expression in developing and mature iNKT, NK, and T cell subsets, we assess the transcriptional basis for NK- iNKT similarities. Our data demonstrate that shared NK- iNKT transcriptional programs are more extensive than currently appreciated and comparable in breadth to those shared by iNKT and MHC-restricted T cells. Finally, we show that the transcriptional patterns expressed constitutively by both NK and iNKT cells represent a core effector program also operational in other innate lymphocytes and induced in adaptive lymphocyte populations following activation.
iNKT cells, like other T lymphocytes, mature in the thymus, where they diverge from MHC-restricted αβ T cells at the CD4+ CD8+ double-positive (DP) stage. iNKT cells subsequently undergo sequential stages of differentiation characterized by differential expression of CD44 and NK1.14 (Supplementary Fig. 1). To characterize the developmental transcriptional programs in iNKT cells in relation to those operating in maturing adaptive αβ T cells, we profiled CD44− NK1.1− (stage 1), CD44+NK1.1− (stage 2) and CD44+NK1.1+ (stage 3) thymic iNKT cells in the context of the ImmGen Project (Fig. 1a). 1850, 155 and 697 genes were differentially expressed from DP to stage 1, stage 1 to stage 2, and stage 2 to stage 3, respectively, using an arbitrary fold change (FC) threshold of FC > 2 (Fig. 1b, c). Transcripts modulated between thymic iNKT cell populations included a number of genes involved in iNKT cell maturation4 as well as several genes of unknown function (Supplementary Tables 1–6). At the DP branch point, we identified a subset of genes modulated selectively by stage 1 iNKT cells but not by early stage CD4+ adaptive T cells (CD4+8int) (Fig. 1d). Although some of these genes such as Zbtb16 (PLZF) and Vdr (vitamin D receptor) are known to play important functions in iNKT cell lineage specification29–31, many have not yet been linked to iNKT cell development (Supplementary Tables 7, 8). At the last iNKT cell maturation stage, many of the most strongly upregulated genes belonged to the killer lectin receptor family, which encodes activating and inhibitory NK receptors (NKRs) (Fig. 1c, bottom panel). By the final stage of ontogeny, iNKT cells expressed several NKR mRNAs at levels comparable to those of splenic NK cells (Supplementary Fig. 2a). In addition, NKR upregulation occurs selectively in developing iNKT cells but not in maturing MHC-restricted T cells or at any stage in early T cell development, with a few exceptions (Fig. 1e). Flow cytometric analysis of thymic iNKT cells confirmed the acquisition of surface NKRs over the course of maturation (Fig. 1f and Supplementary Fig. 2b). K-means clustering analysis determined that a large number of genes follow the same expression kinetics as NKRs over the course of the DP to stage 3 transition (Fig. 1g) suggesting that the progressive upregulation of NKRs may be part of a broader gene program. Together, these data comprehensively characterize shared and distinct gene expression changes occurring in maturing iNKT cells in the broader context of αβ T cell development.
In both mouse and human, differential cytokine production has been reported between CD4+ and CD4−iNKT cells5,32,33. In addition, iNKT cell subsets from the liver have also been suggested to be functionally distinct34. To characterize the transcriptional basis that may underlie subset-specific functional differences, we assessed the gene expression profiles of CD4+ and CD4−iNKT cells sorted from the spleen, liver and lungs of mice. 159 and 261 genes were differentially expressed between CD4+ and CD4−iNKT cells from the spleen or lung, respectively, while only 17 transcripts were differentially expressed between the liver subsets (Fig. 2a, Supplementary Fig. 3 and Supplementary Tables 9–14), suggesting that splenic and pulmonary iNKT cell subsets may be more functionally distinct from each other than are liver iNKT cell subsets. In all three tissues, we found that a number of NKRs were among the most differentially expressed genes, with elevated levels in CD4− compared to CD4+ subsets (Fig. 2a and Supplementary Fig. 3). In liver and spleen, this reflected a larger percentage of NKR-positive cells in CD4− compared to CD4+iNKT cells, as determined by flow cytometry (Fig. 2b). NKR mRNA expression in peripheral iNKT cells, although reduced compared to stage 3 thymic iNKT cells, was maintained at higher levels than most MHC-restricted αβ T cell subsets (Fig. 2c and data not shown). In addition, comparison of liver iNKT cell subsets to their splenic counterparts revealed that CD4− populations are more transcriptionally similar to one another than are CD4+iNKT cells across tissues. A small number of genes were expressed differentially between tissues regardless of CD4 expression (Supplementary Fig. 4), supporting the idea that iNKT cells subsets may perform organ-specific functions.
Because iNKT cells share broad functional features with NK cells, we hypothesized that NKR expression by iNKT cells might reflect a much larger NK-NKT shared transcriptional program than currently appreciated. To assess the extent of transcriptional relatedness between iNKT cells and NK cells at a global level, and to compare the NK- iNKT relationship and with that of iNKT to T cells, we calculated Euclidian distances between subsets of steady-state NK, T and iNKT cells. Euclidian distance, a measure of the similarity between the gene expression patterns of pairwise compared subsets, was determined using the 15% of gene probes with the highest variability among the subsets analyzed, and is displayed as a matrix (Fig. 3). This revealed a strong degree of similarity between mature iNKT and NK cell gene expression (Fig. 3, top panel, area i), contrasting greater distances observed between all MHC-restricted T cells and NK cells (Fig. 3, top panel, area ii). Compared to the iNKT -NK cell distance, iNKT cells exhibited a somewhat closer relationship to memory CD8+ T cells and certain memory CD4+ T cell, however, the latter were sorted using markers expressed at similar levels by iNKT cells (CD44+ and CD62Llow) and likely thus themselves contain a significant percentage of iNKT cells. The average Euclidian distance between iNKT and NK cells was only slightly larger than that separating iNKT cells from naive CD4+ and CD8+ T cells (Fig. 3, top panel, area iii). These relative relationships were maintained when the analysis was performed using all genes and not just the 15% most variable (data not shown). To determine whether the NK- iNKT relationship is dependent on the shared expression of NKRs, we recalculated the distance matrix after removing NKRs and related molecules from the data (see on-line methods). The outcome of the analysis remained essentially unchanged (Supplementary Fig. 5). Thus, the transcriptional relationship between NK and iNKT cells is not limited to the shared expression of NKRs. Together, these data support an unexpectedly substantial transcriptional relationship between iNKT and NK cells that is close in magnitude to that occurring between iNKT and naive T cells.
We next sought to identify the specific concordantly-regulated genes among iNKT, NK and T cells. For this purpose, we performed one-way analysis of variance (ANOVA) comparing the transcriptomes of peripheral steady-state iNKT cells, NK cells and naive and memory CD4+ and CD8+ T cells. Genes with low variability over the entire ImmGen dataset were excluded from the analysis. 20.2% (1192 genes) of the remaining genes were differentially expressed (Bonferroni corrected P value < 0.05) between the three groups. The modulated genes were further classified into 6 categories (see on-line methods): genes expressed similarly in NK and iNKT cells compared to T cells (A1 or A2, higher or lower than in T cells, respectively), genes expressed similarly in iNKT and T cells compared to NK cells (B1 or B2, higher or lower than in NK cells, respectively), and genes expressed differentially in iNKT cells (C1 or C2, higher or lower than T and NK cells, respectively) (Fig. 4a). A number of transcription factors, TCR signaling components, and cytokine or chemokine-related molecules that are known to be expressed differentially by NK, iNKT, and T cells partitioned as expected in the ANOVA categories (Fig. 4b–d). For example, the transcription factor PLZF (Zbtb16, Fig. 4b), the chemokine receptor Cxcr6 (Fig. 4c), and a component of the high-affinity IL-12 receptor (Il12rb1) (Fig. 4d), all known to be expressed at higher levels in iNKT cells than in resting T or NK cells29,30,35,36, partitioned to category C1 (higher in iNKT cells relative to NK and T cells). The transcription factor T-bet (Tbx21, Fig. 4b) as well as Il12rb2 (Fig. 4d), known to be upregulated in NK and iNKT cells37, partitioned to category A1 (higher in iNKT and NK cells relative to T cells). TCR signaling components such as Cd3e, Itk, Plcg1, and Zap70 (Fig. 4c) segregated as anticipated to category B1 (higher in iNKT and T compared to NK, Fig. 4c, d). Functional biological process enrichment analysis using DAVID software38 of category A1 genes showed a statistically significant enrichment for effector functions including NK cell-mediated immunity, chemokine or cytokine responses, signal transduction, and cell motility (Table 1). Distinct biological processes were also enriched in the group of genes upregulated in iNKT cells as compared to NK and MHC-restricted T cells (category C1), and indicated a role for these genes in proliferation and survival, likely reflecting the uniquely activated phenotype of steady state iNKT cells (Table 2).
We found that a comparable number of genes were expressed similarly in iNKT and NK lineages (36.91%, 440 genes, categories A1 and A2) as in iNKT and T cells (43.88%, 523 genes, categories A2 and B2). In addition, about one-fifth of the differentially expressed genes were regulated uniquely in iNKT cells (19.21%, 229 genes, categories C1 and C2) (Fig. 4a, Supplementary Table 15). Thus, in addition to expressing a distinctive genetic program, iNKT cells share a transcriptional program transcriptional with steady-state NK cells that is extensive and similar in magnitude to that shared between iNKT cells and other αβ T cells, consistent with the Euclidian distance analysis.
To determine at what point during thymic development the transcriptional program shared by mature iNKT and NK cells is induced, we analyzed the expression patterns of ANOVA category A1 genes over the course of MHC-restricted T and iNKT cell differentiation by hierarchical clustering. Although approximately 75% of the shared, upregulated NK- iNKT gene program is expressed in early thymic precursors (ETP), these genes are then largely shut-down or downregulated by the DP thymocyte stage, and are not reactivated in differentiating CD4+ or CD8+ T cells. In contrast, differentiating iNKT cells upregulated these same genes, such that stage 3 thymic iNKT cells expressed more than 90% of the program (Fig. 5a). Consistent with this observation, the FC distribution of category A1 genes calculated by comparing the expression of each gene in developing iNKT cells and their DP progenitor showed an increasing shift towards higher FC values with iNKT maturation. All three distributions were significantly different from the baseline FC distribution for all expressed genes as determined by a Kolmogorov-Smirnov (K-S) test, with P values for enrichment of 3.97 × 10−15, 1.28 × 10−19 and 9.14 × 10−48 for stage 1, 2 and 3 iNKT cells respectively (Fig. 5b). In contrast, the FC distribution calculated by comparing naïve splenic CD4+ or CD8+ T cells to DP was not significantly different between the category A1 geneset and all expressed genes (P = 0.79 and P = 0.65, respectively). Conversely to category A1 genes, the genes that were significantly downregulated in mature NK and iNKT cells compared to T cells (ANOVA category A2) were not as strongly elicited in differentiating thymic iNKT cells as in CD4+ and CD8+ T cells (Supplementary Fig. 6). These data extend our earlier observation that iNKT cells acquire NKR expression at the end of development, and indicate that a large part of the transcriptional program iNKT cells share with NK cells is acquired late in thymic maturation.
We next asked if other lymphocytes with innate features might also express this transcriptional program. γδ T cells can be categorized on the basis of their TCR Vγ and Vδ chain usage. The subset bearing the Vγ2 chain tends to be IL-17 polarized, whereas the Vγ2− subsets (including Vγ1.1+Vδ6.3+ and Vγ1.1+Vδ6.3− subsets) predominantly secrete TH1 or TH2 cytokines39. Distinct intraepithelial lymphocyte (IEL) CD8αα+ γδ T cell populations posses an effector phenotype similar to that of iNKT cells40,41.
In splenic γδ T cells negative for the activation marker CD44, NK- iNKT shared genes (ANOVA category A1) were expressed at relatively low levels, similar to those observed in resting T cells. A subset of these genes was upregulated in splenic CD44+ Vγ2+ and Vγ2− cells. Strikingly, we found that all tissue-resident IEL γδ T cells expressed a large cluster of category A1 genes at levels surpassing those of the reference NK and iNKT populations (Fig. 6a). FC distributions comparing the expression of category A1 genes in splenic or IEL γδ T cells and T cells revealed an enrichment of over-expressed genes (P value 4.67 × 10−41 and 3.90 × 10−34, respectively) (Fig. 6b). IEL γδ T cells expressed an even smaller proportion of ANOVA category A2 genes (downregulated in NK and iNKT compared to T) than did iNKT cells, although most of these genes were expressed in splenic γδ T cells (data not shown). Although less prominently than for the shared NK- iNKT cell program (category A1) a portion of the genes upregulated in iNKT cells but not T or NK subsets (category C1) were also relatively highly expressed in γδ T cell subsets (Supplementary Fig. 7a). Together, these data suggest that most of the gene program shared by NK and iNKT cells, as well as part of the program differentially upregulated in iNKT cells, is also utilized by populations of γδ T cells at steady state.
We next investigated the expression of the shared NK- iNKT transcriptional program in CD8+ effector T cells, a cell population that shares functional characteristics with NK and iNKT cells, including the expression of certain NKRs42. We examined the expression of ANOVA categories A1 and A2 in CD8+ T cells from the spleen of ovalbumin (OVA)-reactive αβ TCR transgenic (OT-I) mice following infection with Listeria monocytogenes expressing OVA. Only a small fraction of ANOVA category A1 genes became upregulated in effector CD8+ T cells at 12, 24, and 48 h following Listeria infection. By day 6, however, the NK- iNKT shared program was dramatically upregulated, with a FC distribution significantly enriched for higher expression, and this genetic program was maintained in CD8+ memory T cells as late as day 100 following infection (Fig. 7a, b). In contrast, only a limited portion of category C1 genes (differentially expressed by iNKT cells) followed a similar pattern in effector CD8+ T cell populations (Supplementary Fig. 7b). The genes downregulated in NK and iNKT cells compared to T cells (ANOVA category A2, which by definition are widely expressed in naive OT-I transgenic CD8+ T cells), were partially repressed following infection (Supplementary Fig. 8). Similar results were obtained when the expression of the NK- iNKT shared transcriptional programs were examined in CD8+ T cells from OT-I transgenic mice infected with OVA-expressing vesicular stomatitis virus (VSV) (Supplementary Fig. 9). We found that the homologous human genes comprising the NK- iNKT shared program (ANOVA category A1) were also expressed at significantly higher levels in human peripheral blood effector memory CD8+ T cell populations as compared to naive CD8+ T cells43 (Fig. 7c, d). Thus, a large proportion of the genetic program shared by NK and iNKT cells is elicited in effector αβ T cells, but only several days following their activation.
iNKT cells do not fit the classical paradigm of adaptive T cell immunity. Indeed, innate features are at the core their physiological functions3,14. The transcriptional basis for these features, however, remains incompletely defined.
The transcriptional programs and regulatory factors we found to be operating during iNKT cell maturation were consistent with previous reports, and included Zbtb16 (PLZF), Vdr, Tbx21 (Tbet), components of the NF-κB and Ras-MapK pathways, and NKRs4,37,44,45. By comparing developing iNKT cells to differentiating MHC-restricted T cells, we define transcriptional programs expressed specifically by stage 1 iNKT cells shortly after the DP branch point. We thus highlight a large number genes not previously known to affect iNKT cell biology but likely modulating iNKT cell thymic maturation specifically.
A number of genes reported to be involved in iNKT cell development were not expressed in an iNKT cell specific-manner. For instance, NF-κB and Ras/MapK pathway members such as NFkb1, RelB, Ras/MapK and Egr2 were upregulated in both iNKT and non-iNKT thymocyte populations4 (data not shown). Other genes known to affect iNKT cells ontogeny, Bcl11b46 and the chromatin modifier Med147 for example, exhibited little or no transcriptional variation over the course of iNKT cell differentiation. The functional regulation of these and other genes not detected in our analyses may thus be controlled at post-transcriptional levels.
Mature iNKT cells share several innate functional features with NK cells, developmentally distant relatives. We hypothesized that NK and iNKT cells shared a broader transcriptional program than is currently appreciated. Our analyses revealed that the transcriptional patterns iNKT cells share with NK cells make up nearly as large a part of the iNKT transcriptome as those shared with MHC-restricted T cells. Further, we found that the NK- iNKT cell shared program was also active in steady-state γδ T cells subsets similarly poised for rapid, innate-like responsiveness. The NK- iNKT program can in addition be induced in adaptive αβ T cells, but only days following antigen-specific stimulation. These data suggest that the NK- iNKT shared program represents a core effector program operational in lymphocytes of distinct lineages, consistent with the many functional capabilities iNKT, NK and activated MHC-restricted T cells have in common.
The factors that regulate expression of this shared effector program remain to be fully defined. Nevertheless, our data provides several leads. Acquired largely at the end of thymic iNKT cell maturation, the shared program mirrors the cells’ acquisition of NKRs. NKR expression is driven by the transcription factor T-bet both in NK and iNKT cells37. Thus, it is likely that T-bet, which we found to be among the genes significantly upregulated in both NK and iNKT compared to resting T cells, plays an important role in eliciting and maintaining at least part of the shared effector lymphocyte program. T-bet-deficient iNKT cells express reduced mRNA and protein levels of IFN-γ, granzyme B, Fas ligand, CCR5, and CD38, all molecules that are part of the shared program44. IL-15, recently reported to act upstream of T-bet in maturing iNKT cells45, is also likely to be involved in iNKT cell acquisition of this program. Furthermore, IL-15 and T-bet are important for regulating CD8+ effector T cell responses48 as well as for the homeostasis of IEL γδ T cells49. Thus, IL-15 and T-bet may play an important role in inducing the expression of the shared effector program in several cell types.
We also highlight a number of previously unappreciated transcriptional regulators exhibiting similar expression patterns in NK and iNKT cells, and that may help regulate the core effector program in innate lymphocytes. For example, the transcription factor Bhlhe40, a circadian rhythm regulator50 possessing immune-modulatory functions in CD4+ T cells51,52, as well as Smad3, a transcription factor important for tuning TGF-β-mediated lymphocyte activation, were upregulated in both NK and iNKT cells compared to resting T cells. Also, a number of factors were downregulated in NK and iNKT cells. Among these genes were Lef1 and Tcf7, transcription factors downstream of the Wnt signaling pathway that are important for the establishment and maintenance of T cell identity53. Wnt signaling, which is typically repressed upon T cell activation54,55. The relatively low expression of Tcf7 and Lef1 in NK and iNKT cells during development is consistent with the acquisition their terminally differentiated effector phenotype at steady state.
Approximately 20% of the significantly differentially expressed genes between NK, iNKT, and T cells exhibited transcriptional patterns specific to iNKT cells. As expected, PLZF (Zbtb16) and Gata-3 were among these factors. The genes preferentially upregulated in iNKT cells (category C1 genes) were enriched for cellular activation and survival programs. For instance, several AP-1 family members (Jun, Junb, Jund, Fos, Fosb and Cebpb) normally induced in cells only after activation, were expressed relatively highly in iNKT cells. Constitutive expression of AP-1 transcription factors by iNKT cells is consistent with their poised effector phenotype.
Together, these data offer a new view of iNKT cells and their relationships to other lymphocyte lineages. Using the Immunological Genome consortium database, we have uncovered gene expression programs that both link to and differentiate iNKT cells from other innate and adaptive lymphocytes. Our data highlight extensive genetic modules that are shared with NK cells and other innate-like T cells, and that are also elicited several days following activation in adaptive T cells. By defining both distinct and shared transcriptional programs in iNKT cells, our data opens avenues for future research and brings into clearer focus how lymphocyte populations that differ markedly in their ontogeny can ultimately carry out similar effector functions through modular expression of similar transcriptional programs.
6 week old C57BL/6 male mice shipped from Jackson Laboratories one week prior to organ harvest were used. Mice were maintained under specific pathogen free conditions. All studies were approved by the Animal Care and Use Committee of the Dana-Farber Cancer Institute.
Anti-CD19 (MB19-1), anti-B220 (RA3–6B2), anti-Ter119 (TER119), anti-CD11b (M1/70), anti-CD11c (N418), anti-Ly6G/Gr1 (A18), anti-CD8α (53/6.7), anti-TCRβ (H58–597), anti-NK1.1 (PK136), anti-CD44 (IM7), anti-CD4 (GK1.5), anti-CD45 (102), anti-CD3ε (145–2C11), anti-Ly49e,f (CM4), anti-NKG2D (CX5), anti-NKG2A (16a11), anti-2B4 (ebio244F4), and anti-CD16,32 (2.4G2) were from eBioscience. Anti-Ly49a (A1) and anti-Ly49c,1 (5F6) were from BD biosciences. CD1d-tetramers loaded with PBS-57 (an α-galactosylceramide analog) were provided by the NIH tetramer facility.
All immune cells purification were performed in strict adherence to the ImmGen standard Operating Procedure guidelines (available at www.immgen.org). Further details on thymic, NK cell, γδ T cell, and CD8+ T cell populations used for comparison to iNKT cells can be found at the ImmGen website. For iNKT cells, thymocytes, splenocytes, liver and lung mononuclear cells were isolated from 5–10 mice per sample. Thymocytes were disaggregated, blocked with anti-CD16 and CD32 (clone 2.4G2), then stained with fluorophore-labeled antibodies for depletion of non- iNKT cell populations, and separated with anti-fluorophore magnetic beads (Miltenyi). Spleens were disaggregated, treated with ACK lysis buffer (Lonza) to remove red blood cells, and then depleted of non- iNKT cells as above. Lungs and livers were harvested after perfusion with cold PBS and mechanically homogenized. Lungs were digested for 15 minutes at 37° C in 7 U/ml of Liberase III enzyme (Roche) in DMEM, then filtered and washed. Livers were homogenized and liver mononuclear cells (LMNC) were isolated by Ficoll density gradient centrifugation. iNKT cell-enriched thymocytes, splenocytes, LMNCs and lung cells were stained for cell surface markers (see www.immgen.org for complete staining procedure including gating strategy) and double sorted directly into Trizol (Invitrogen) at a purity of >99%. By staining thymocytes from CD1d-deficient mice that lack iNKT cells, a false positive rate of 2.7 ± 0.9% was estimated in the case of CD44− NK1.1− (Stage 1) iNKT cells, the rarest thymic subset. Contamination was negligible for other iNKT cell populations. Two to four replicates were obtained for each sample using a FACSAria, with the exception of the CD4−iNKT subset from the lung, for which only a single replicate passed quality control (see below). RNA extraction, microarray hybridization (Affymetrix MoGene 1.0 ST array) and data processing were performed at the ImmGen processing center. For further details, please see Supplementary Table 16 (subset nomenclature key), the Data Generation and Quality Control pipeline documentation, the ImmGen Quality Control Statistics or the ImmGen website (www.immgen.org). Non-ImmGen human and mouse datasets were downloaded from NCBI Geo datasets43,56. Human homologs of mouse genes were determined using NCBI HomoloGene.
Data from the March 2011 ImmGen release (802 arrays with 22,268 probesets) were used. Probesets associated with the same gene symbol were consolidated by selecting the probeset with the highest mean expression overall. For heatmaps, data were log2-transformed and a relative color scale with row centering (subtraction of the mean) and normalization was used. Heatmaps were produced by using the HeatmapViewer module of GenePattern (www.broadinstitute.org/cancer/software/genepattern/). When indicated, Pearson correlation with pair-wise complete linkage was applied to rows for clustering analysis. Volcano plots were produced by using the Multiplot module of GenePattern.
For K-means clustering analysis, genes were pre-filtered for mean expression value ≥ 120 (cut-off above which genes have a 95% chance of expression) and for FC>2 between any two subsets analyzed. Clustering was performed using the ExpressCluster application (Scott Davis, Harvard Medical School, Boston MA – application and documentation available at http://cbdm.hms.harvard.edu) with K=10.
For Euclidian distance matrices, the 15% most variable genes were identified using the PopulationDistances PCA application (Scott Davis), which filters probes based on a variation of ANOVA analysis using the geometric standard deviation of populations to weight genes that vary in multiple populations. The selected genes were log2-transformed, filtered for probes with a mean expression value ≥ 120, and mean centered prior to visualization. Activating and inhibitory NKRs, adaptors and signaling partners (Klra1, Klra2, Klra3, Klra5, Klra6, Klra8, Klra9, Klra10, Klra17, Klrb1a, Klrb1b, Klrb1c, Klrb1f, Klrc1, Klrc2, Klrc3, Klrd1, Klre1, Klri1, Klri2, Klrk1, Klrg2, Ncr1, Cd244, Fcer1g, Tyrobp and Hcst) were present in the initial gene list used in the first analysis (Fig. 3, top panel) and were manually removed as indicated for the second analysis (Fig. 3, bottom panel). Klra1, Klra3, Klra5, Klra6, Klra8, Klra9, Klra10, Klrb1a, Klrb1b, Klrb1c, Klrb1f, Klrc1, Klrc2, Klrc3, Klrd1, Klre1, Klri1, Klri2, Klrk1, Ncr1, Cd244, Fcer1g, Tyrobp (but not Klra2, Klra17, Klrg2 or Hcst) were present after filtering for 15% most variable gene list and were contributed to the first, but not the second Euclidian distance analysis.
For ANOVA, data were log2-transformed, low-variability genes with a standard deviation of ≤ 0.5 across all ImmGen samples were removed and only genes with expression values ≥ 120 in 2 or more arrays were considered, leaving 5,900 genes. The ANOVA was performed using the Matlab (MathWorks) function “anova1” to compare iNKT, NK and T cell populations Bonferroni correction was applied to the resulting list of P values, and 1192 genes passed the ANOVA (p < 0.05 out of 5,900). A secondary test was used to determine which of the three populations significantly differed from the other two, as indicated by the “multcompare” function of Matlab. Based on the results of this analysis, the 1,192 genes were then separated into 14 gene categories. By comparing the average expression levels in NK, iNKT, and T cells for each gene, these categories were then sorted into 6 groups of genes: genes expressed most similarly by NK and iNKT cells (either A1, up- or A2, downregulated compared to T), genes expressed most similarly in T and iNKT (either B1, up- or B2, downregulated compared to NK) and genes expressed uniquely in iNKT (either C1, up- or C2, downregulated compared to NK and T). Functional geneset enrichment analysis was performed using DAVID software (Version 6.7, National Institutes of Health, National Institute for Allergy and Infectious Diseases)57. Panther biological processes38 are shown in Tables 1 and 2. P values calculated by DAVID represent a modified Fisher Exact test. Biological processes with P values less than 0.01 are shown.
For estimation of the significance of enrichment for the FC distributions associated with the heatmaps in Figs. 5–7, Kolmogorov-Smirnov P values were calculated with JMP (SAS Institute) comparing the selected geneset to all genes meeting the criteria for expression (>120) in the samples tested.
We thank the NIH tetramer facility for their ongoing support. We thank M. Painter, C. Benoist, S. Raychaudhuri, X. Hu, J. Erricson, S. Davis, H. Li, T. Kreslavsky, L. Lanier and A. Goldrath for advice, discussions and technical assistance. The work was supported by R01AI063428 to M.B.B., T32AI007306 to P.J.B, and R24AI072073 to the ImmGen consortium.
|1.||Heng TS,Painter MW,Immunological Genome Project C. The Immunological Genome Project: networks of gene expression in immune cellsNat ImmunolYear: 200891091109418800157|
|2.||Cohen NR,Garg S,Brenner MB. Antigen Presentation by CD1 Lipids, T Cells, and NKT Cells in Microbial ImmunityAdv ImmunolYear: 200910219419477319|
|3.||Bendelac A,Savage PB,Teyton L. The biology of NKT cellsAnnu Rev ImmunolYear: 20072529733617150027|
|4.||Godfrey DI,Stankovic S,Baxter AG. Raising the NKT cell familyNat ImmunolYear: 20101119720620139988|
|5.||Coquet JM,et al. Diverse cytokine production by NKT cell subsets and identification of an IL-17-producing CD4-NK1.1- NKT cell populationProc Natl Acad Sci U S AYear: 2008105112871129218685112|
|6.||Cerundolo V,Silk JD,Masri SH,Salio M. Harnessing invariant NKT cells in vaccination strategiesNat Rev ImmunolYear: 20099283819079136|
|7.||Makino Y,Kanno R,Ito T,Higashino K,Taniguchi M. Predominant expression of invariant V alpha 14+ TCR alpha chain in NK1.1+ T cell populationsInt ImmunolYear: 19957115711618527413|
|8.||Bendelac A. Mouse NK1+ T cellsCurr Opin ImmunolYear: 199573673747546402|
|9.||Godfrey DI,MacDonald HR,Kronenberg M,Smyth MJ,Van Kaer L. NKT cells: what's in a name?Nat Rev ImmunolYear: 2004423123715039760|
|10.||Gregoire C,et al. The trafficking of natural killer cellsImmunol RevYear: 200722016918217979846|
|11.||Matsuda JL,et al. Tracking the response of natural killer T cells to a glycolipid antigen using CD1d tetramersJ Exp MedYear: 200019274175410974039|
|12.||Cooper MA,et al. In vivo evidence for a dependence on interleukin 15 for survival of natural killer cellsBloodYear: 20021003633363812393617|
|13.||Matsuda JL,et al. Homeostasis of V alpha 14i NKT cellsNat ImmunolYear: 2002396697412244311|
|14.||Brigl M,Brenner MB. How invariant natural killer T cells respond to infection by recognizing microbial or endogenous lipid antigensSemin ImmunolYear: 201022798619948416|
|15.||Lanier LL. Evolutionary struggles between NK cells and virusesNat Rev ImmunolYear: 2008825926818340344|
|16.||Kuylenstierna C,et al. NKG2D performs two functions in invariant NKT cells: direct TCR-independent activation of NK-like cytolysis and co-stimulation of activation by CD1dEur J ImmunolYear: 2011411913192321590763|
|17.||Kawamura T,et al. NKG2A inhibits invariant NKT cell activation in hepatic injuryJ ImmunolYear: 200918225025819109156|
|18.||Maeda M,Lohwasser S,Yamamura T,Takei F. Regulation of NKT cells by Ly49: analysis of primary NKT cells and generation of NKT cell lineJ ImmunolYear: 20011674180418611591738|
|19.||Ota T,et al. IFN-gamma-mediated negative feedback regulation of NKT-cell function by CD94/NKG2BloodYear: 200510618419215746081|
|20.||Brennan PJ,et al. Invariant natural killer T cells recognize lipid self antigen induced by microbial danger signalsNat ImmunolYear: 2011121202121122037601|
|21.||Paget C,et al. Activation of invariant NKT cells by toll-like receptor 9-stimulated dendritic cells requires type I interferon and charged glycosphingolipidsImmunityYear: 20072759760917950005|
|22.||Salio M,et al. Modulation of human natural killer T cell ligands on TLR-mediated antigen-presenting cell activationProc Natl Acad Sci U S AYear: 2007104204902049518077358|
|23.||Reschner A,Hubert P,Delvenne P,Boniver J,Jacobs N. Innate lymphocyte and dendritic cell cross-talk: a key factor in the regulation of the immune responseClin Exp ImmunolYear: 200815221922618336590|
|24.||Andrews DM,Scalzo AA,Yokoyama WM,Smyth MJ,Degli-Esposti MA. Functional interactions between dendritic cells and NK cells during viral infectionNat ImmunolYear: 2003417518112496964|
|25.||Brigl M,Bry L,Kent SC,Gumperz JE,Brenner MB. Mechanism of CD1d-restricted natural killer T cell activation during microbial infectionNat ImmunolYear: 200341230123714578883|
|26.||Fernandez NC,et al. Dendritic cells directly trigger NK cell functions: cross-talk relevant in innate anti-tumor immune responses in vivoNat MedYear: 1999540541110202929|
|27.||Vincent MS,et al. CD1-dependent dendritic cell instructionNat ImmunolYear: 200231163116812415264|
|28.||Walzer T,Dalod M,Vivier E,Zitvogel L. Natural killer cell-dendritic cell crosstalk in the initiation of immune responsesExpert Opin Biol TherYear: 20055Suppl 1S49S5916187940|
|29.||Savage AK,et al. The transcription factor PLZF directs the effector program of the NKT cell lineageImmunityYear: 20082939140318703361|
|30.||Kovalovsky D,et al. The BTB-zinc finger transcriptional regulator PLZF controls the development of invariant natural killer T cell effector functionsNat ImmunolYear: 200891055106418660811|
|31.||Yu S,Cantorna MT. The vitamin D receptor is required for iNKT cell developmentProc Natl Acad Sci U S AYear: 20081055207521218364394|
|32.||Gumperz JE,Miyake S,Yamamura T,Brenner MB. Functionally distinct subsets of CD1d-restricted natural killer T cells revealed by CD1d tetramer stainingJ Exp MedYear: 200219562563611877485|
|33.||Watarai H,et al. Development and function of invariant natural killer T cells producing T(h)2- and T(h)17-cytokinesPLoS BiolYear: 201210e100125522346732|
|34.||Crowe NY,et al. Differential antitumor immunity mediated by NKT cell subsets in vivoJ Exp MedYear: 20052021279128816275765|
|35.||Brigl M,et al. Innate and cytokine-driven signals, rather than microbial antigens, dominate in natural killer T cell activation during microbial infectionJ Exp MedYear: 20112081163117721555485|
|36.||Johnston B,Kim CH,Soler D,Emoto M,Butcher EC. Differential Chemokine Responses and Homing Patterns of Murine TCRalphabeta NKT Cell SubsetsJ ImmunolYear: 20031712960296912960320|
|37.||Townsend MJ,et al. T-bet regulates the terminal maturation and homeostasis of NK and Valpha14i NKT cellsImmunityYear: 20042047749415084276|
|38.||Thomas PD,et al. PANTHER: a browsable database of gene products organized by biological function, using curated protein family and subfamily classificationNucleic Acids ResYear: 20033133434112520017|
|39.||O'Brien RL,Born WK. gammadelta T cell subsets: a link between TCR and function?Semin ImmunolYear: 20102219319820451408|
|40.||Fahrer AM,et al. Attributes of gammadelta intraepithelial lymphocytes as suggested by their transcriptional profileProc Natl Acad Sci U S AYear: 200198102611026611526237|
|41.||Shires J,Theodoridis E,Hayday AC. Biological insights into TCRgammadelta+ and TCRalphabeta+ intraepithelial lymphocytes provided by serial analysis of gene expression (SAGE)ImmunityYear: 20011541943411567632|
|42.||Vivier E,Anfossi N. Inhibitory NK-cell receptors on T cells: witness of the past, actors of the futureNat Rev ImmunolYear: 2004419019815039756|
|43.||Gattinoni L,et al. A human memory T cell subset with stem cell-like propertiesNat MedYear: 2011171290129721926977|
|44.||Matsuda JL,et al. T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cellsBloodYear: 20061072797280516357323|
|45.||Gordy LE,et al. IL-15 regulates homeostasis and terminal maturation of NKT cellsJ ImmunolYear: 20111876335634522084435|
|46.||Kastner P,et al. Bcl11b represses a mature T-cell gene expression program in immature CD4(+)CD8(+) thymocytesEur J ImmunolYear: 2010402143215420544728|
|47.||Yue X,Izcue A,Borggrefe T. Essential role of Mediator subunit Med1 in invariant natural killer T-cell developmentProc Natl Acad Sci U S AYear: 2011108171051711021949387|
|48.||Sullivan BM,Juedes A,Szabo SJ,von Herrath M,Glimcher LH. Antigen-driven effector CD8 T cell function regulated by T-betProc Natl Acad Sci U S AYear: 2003100158181582314673093|
|49.||Inagaki-Ohara K,Nishimura H,Mitani A,Yoshikai Y. Interleukin-15 preferentially promotes the growth of intestinal intraepithelial lymphocytes bearing gamma delta T cell receptor in miceEur J ImmunolYear: 199727288528919394814|
|50.||Honma S,et al. Dec1 and Dec2 are regulators of the mammalian molecular clockNatureYear: 200241984184412397359|
|51.||Miyazaki K,et al. The role of the basic helix-loop-helix transcription factor Dec1 in the regulatory T cellsJ ImmunolYear: 20101857330733921057086|
|52.||Sun H,Lu B,Li RQ,Flavell RA,Taneja R. Defective T cell activation and autoimmune disorder in Stra13-deficient miceNat ImmunolYear: 200121040104711668339|
|53.||Weber BN,et al. A critical role for TCF-1 in T-lineage specification and differentiationNatureYear: 2011476636821814277|
|54.||Willinger T,et al. Human naive CD8 T cells down-regulate expression of the WNT pathway transcription factors lymphoid enhancer binding factor 1 and transcription factor 7 (T cell factor-1) following antigen encounter in vitro and in vivoJ ImmunolYear: 20061761439144616424171|
|55.||Gattinoni L,et al. Wnt signaling arrests effector T cell differentiation and generates CD8+ memory stem cellsNat MedYear: 20091580881319525962|
|56.||Marshall HD,et al. Differential expression of Ly6C and T-bet distinguish effector and memory Th1 CD4(+) cell properties during viral infectionImmunityYear: 20113563364622018471|
|57.||Huang da W,Sherman BT,Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNat ProtocYear: 20094445719131956|
Previous Document: Size-controlled synthesis and morphology evolution of bismuth trifluoride nanocrystals via a novel s...
Next Document: The psoriasis-associated D10N variant of the adaptor Act1 with impaired regulation by the molecular ...