| Shape change in human platelets: measurement with a channelyzer and visualisation by electron microscopy. | |
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MedLine Citation:
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PMID: 10938878 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The increase in median platelet volume (MPV) that occurs following the addition of various agonists can be recorded by high-resolution channelyzers. However, this increase in MPV may not represent the platelet shape change (PSC), an early phase of platelet activation characterised by the formation of pseudopodia. We therefore compared the MPV after adding various agonists (serotonin, ADP or a thromboxane A2 analogue) with scanning electron micrographs (EM) of the platelets in the same sample. Saline alone did not increase the MPV or alter platelet morphology. In contrast, the selected agonists altered both the MPV and EM. Adrenaline (5.0 mumol/l) alone did not influence either of these indices of platelet activation. However, when a lower concentration of adrenaline (0.2 mumol/l) was added in combination with a dose of serotonin (0.02 mumol/l) that did not affect the MPV on its own, the measured MPV increased significantly and the EM showed pseudopodia formation. Because the channelyzer method is reproducible and suited to serial measurements of the MPV, it may become a useful tool for the investigation of platelet activation. |
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Authors:
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I A Jagroop; I Clatworthy; J Lewin; D P Mikhailidis |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Platelets Volume: 11 ISSN: 0953-7104 ISO Abbreviation: Platelets Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-09-14 Completed Date: 2000-09-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9208117 Medline TA: Platelets Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 28-32 Citation Subset: IM |
Affiliation:
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Department of Molecular Pathology and Clinical Biochemistry, Royal Free & University College Medical School (University of London), UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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pharmacology Adenosine Diphosphate / pharmacology Blood Platelets / cytology*, drug effects Cell Size / drug effects, physiology Epinephrine / pharmacology Humans Microscopy, Electron Platelet Count / instrumentation, methods Platelet Function Tests / instrumentation, methods Pseudopodia / drug effects Serotonin / pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
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50-67-9/Serotonin; 51-43-4/Epinephrine; 58-64-0/Adenosine Diphosphate; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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