Document Detail


Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity, and line tension.
MedLine Citation:
PMID:  17951747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Membrane shape parameters such as curvature, bending elasticity, and lateral tension, are relevant to the lateral organization and function of biomembranes, and may critically influence the formation of lateral clustering patterns observed in living cells. Fluorescence laser-scanning microscopy can be used to image vesicles and cell membranes, and from shape analysis of these images mechanical membrane parameters can be quantified. Methods to analyze images of equatorial sections obtained by confocal or multiphoton microscopy are detailed, in order to estimate curvature, lateral tension, line tension, relative differences in mean curvature and Gaussian curvature bending moduli, and fluorescence dye intensity profiles, typically within coexisting liquid-ordered and liquid-disordered membrane domains. A variety of shape tracing and shape fitting methods are compared.
Authors:
Samuel T Hess; Manasa V Gudheti; Michael Mlodzianoski; Tobias Baumgart
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  400     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-12-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  367-87     Citation Subset:  IM    
Affiliation:
Chemistry Department, University of Pennsylvania, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / chemistry*,  metabolism
Elasticity
Image Processing, Computer-Assisted*
Membranes, Artificial*
Microscopy, Confocal
Phase Transition*
Surface Tension
Grant Support
ID/Acronym/Agency:
1-K25-AI-65459-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Membranes, Artificial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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