| Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity, and line tension. | |
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MedLine Citation:
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PMID: 17951747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Membrane shape parameters such as curvature, bending elasticity, and lateral tension, are relevant to the lateral organization and function of biomembranes, and may critically influence the formation of lateral clustering patterns observed in living cells. Fluorescence laser-scanning microscopy can be used to image vesicles and cell membranes, and from shape analysis of these images mechanical membrane parameters can be quantified. Methods to analyze images of equatorial sections obtained by confocal or multiphoton microscopy are detailed, in order to estimate curvature, lateral tension, line tension, relative differences in mean curvature and Gaussian curvature bending moduli, and fluorescence dye intensity profiles, typically within coexisting liquid-ordered and liquid-disordered membrane domains. A variety of shape tracing and shape fitting methods are compared. |
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Authors:
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Samuel T Hess; Manasa V Gudheti; Michael Mlodzianoski; Tobias Baumgart |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 400 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2007-12-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 367-87 Citation Subset: IM |
Affiliation:
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Chemistry Department, University of Pennsylvania, PA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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chemistry*,
metabolism Elasticity Image Processing, Computer-Assisted* Membranes, Artificial* Microscopy, Confocal Phase Transition* Surface Tension |
| Grant Support | |
ID/Acronym/Agency:
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1-K25-AI-65459-01/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membranes, Artificial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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