| Shaking of the intact rat and intestinal angulation diminish the jejunal unstirred layer. | |
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MedLine Citation:
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PMID: 1426864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sizeable pre-epithelial diffusion barrier (unstirred layer) is present during perfusion of the rat jejunum. In the present study, three rapidly transported compounds, CO, [14C]warfarin, and glucose (5.5 mmol/L), were used as probes to assess the ability of manipulations to reduce the unstirred layer. This layer was 700-800 microns thick in a 30-cm jejunal segment perfused in conventional fashion on the abdominal wall. Placement of four sharp angulations in the segment or replacement in the abdominal cavity reduced the maximal unstirred layer to 200-400 microns. Increasingly rapid shaking of the anesthetized, intact rat on a platform shaker produced progressively thinner unstirred layers. At 250 revolutions per minute, the maximal layer ranged from 32 to 68 microns for the three probes and may have been appreciably less if the epithelium offered appreciable resistance. Shaking yields a > 15-fold reduction in unstirred layer resistance and provides a means for measuring this resistance and for obtaining more accurate assessment of the true in vivo transport Michaelis constant (Km) of any compound. |
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Authors:
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M D Levitt; J K Furne; D G Levitt |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 103 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-12-14 Completed Date: 1992-12-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1460-6 Citation Subset: AIM; IM |
Affiliation:
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Research Service, Veterans Administration Medical Center, Minneapolis, Minnesota. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active Carbon Monoxide / pharmacokinetics Glucose / pharmacokinetics Intestinal Absorption / physiology* Jejunum / anatomy & histology, metabolism* Motion* Rats Rats, Sprague-Dawley Warfarin / pharmacokinetics |
| Grant Support | |
ID/Acronym/Agency:
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5R01-DK13309/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose; 630-08-0/Carbon Monoxide; 81-81-2/Warfarin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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