Document Detail

Shadow masking for nanomaterial-based biosensors incorporated with a microfluidic device.
MedLine Citation:
PMID:  23420190     Owner:  NLM     Status:  MEDLINE    
Integrating PDMS channels and chips containing pre-functionalized biosensors into microfluidic devices with irreversible sealing has been challenging because the integration process requires use of an O2 plasma treatment that usually destroys the biosensors. In this study, we examined the usefulness of introducing a shadow mask into the process as a method of protecting the pre-functionalized biosensors. Single nanowire sensors were pre-functionalized with fluorescently labeled biomolecules and then subjected to O2 plasma with and without the shadow mask. Results from the two groups were then compared. Those sensors without a shadow mask were destroyed, giving the sensor an infinite resistance and reduced fluorescence intensity. In contrast, the sensors with the shadow mask were protected and exhibited little changes in the resistance and fluorescence intensity. Then two different nanowires, aptamers incorporated polypyrrole nanowire (entrapment) and antibodies immobilized polyaniline nanowire (surface covalent binding), were used to investigate their detection performance before and after the plasma treatment in the presence of shadow mask. The protected samples showed a good sensitivity to the targets with a slight reduction in response compared with the as-prepared samples. After the O2 plasma treatment, the microfluidic channels were integrated with single nanowire biosensors. This microfluidic biosensor showed a high sensitivity with about ~0.5 % change in conductance at the lowest IgE protein concentration of 10 pM.
Jiyong Huang; Innam Lee; Xiliang Luo; Xinyan Tracy Cui; Minhee Yun
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biomedical microdevices     Volume:  15     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-10     Completed Date:  2014-01-13     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  531-7     Citation Subset:  IM    
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MeSH Terms
Biosensing Techniques / instrumentation*
Fluorescent Dyes / chemistry
Immunoglobulin E / analysis
Immunoglobulin G / analysis
Microfluidic Analytical Techniques / instrumentation*
Oxygen / chemistry
Polymers / chemistry
Pyrroles / chemistry
Grant Support
1R21B008825//PHS HHS; R21 EB008825/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Fluorescent Dyes; 0/Immunoglobulin G; 0/Polymers; 0/Pyrroles; 30604-81-0/polypyrrole; 37341-29-0/Immunoglobulin E; S88TT14065/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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